1cz7
From Proteopedia
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| - | [[Image:1cz7.gif|left|200px]] | + | [[Image:1cz7.gif|left|200px]] |
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| - | '''THE CRYSTAL STRUCTURE OF A MINUS-END DIRECTED MICROTUBULE MOTOR PROTEIN NCD REVEALS VARIABLE DIMER CONFORMATIONS''' | + | {{Structure |
| + | |PDB= 1cz7 |SIZE=350|CAPTION= <scene name='initialview01'>1cz7</scene>, resolution 2.9Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> and <scene name='pdbligand=ADP:ADENOSINE-5'-DIPHOSPHATE'>ADP</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
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| + | '''THE CRYSTAL STRUCTURE OF A MINUS-END DIRECTED MICROTUBULE MOTOR PROTEIN NCD REVEALS VARIABLE DIMER CONFORMATIONS''' | ||
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==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1CZ7 is a [ | + | 1CZ7 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CZ7 OCA]. |
==Reference== | ==Reference== | ||
| - | The crystal structure of the minus-end-directed microtubule motor protein ncd reveals variable dimer conformations., Kozielski F, De Bonis S, Burmeister WP, Cohen-Addad C, Wade RH, Structure. 1999 Nov 15;7(11):1407-16. PMID:[http:// | + | The crystal structure of the minus-end-directed microtubule motor protein ncd reveals variable dimer conformations., Kozielski F, De Bonis S, Burmeister WP, Cohen-Addad C, Wade RH, Structure. 1999 Nov 15;7(11):1407-16. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10574799 10574799] |
[[Category: Drosophila melanogaster]] | [[Category: Drosophila melanogaster]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: MG]] | [[Category: MG]] | ||
[[Category: kinesin superfamily]] | [[Category: kinesin superfamily]] | ||
| - | [[Category: microtubule | + | [[Category: microtubule motor]] |
[[Category: ncd crystal structure]] | [[Category: ncd crystal structure]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:30:40 2008'' |
Revision as of 08:30, 20 March 2008
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| , resolution 2.9Å | |||||||
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| Ligands: | and | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
THE CRYSTAL STRUCTURE OF A MINUS-END DIRECTED MICROTUBULE MOTOR PROTEIN NCD REVEALS VARIABLE DIMER CONFORMATIONS
Overview
BACKGROUND: The kinesin superfamily of microtubule-associated motor proteins are important for intracellular transport and for cell division in eukaryotes. Conventional kinesins have the motor domain at the N terminus of the heavy chain and move towards the plus end of microtubules. The ncd protein is necessary for chromosome segregation in meiosis. It belongs to a subfamily of kinesins that have the motor domain at the C terminus and move towards the minus end of microtubules. RESULTS: The crystal structure of dimeric ncd has been obtained at 2.9 A resolution from crystals with the C222(1) space group, with two independent dimers per asymmetric unit. The motor domains in these dimers are not related by crystallographic symmetry and the two ncd dimers have significantly different conformations. An alpha-helical coiled coil connects, and interacts with, the motor domains. CONCLUSIONS: The ncd protein has a very compact structure, largely due to extended interactions of the coiled coil with the head domains. Despite this, we find that the overall conformation of the ncd dimer can be rotated by as much as 10 degrees away from that of the twofold-symmetric archetypal ncd. The crystal structures of conventional kinesin and of ncd suggest a structural rationale for the reversal of the direction of movement in chimeric kinesins.
About this Structure
1CZ7 is a Single protein structure of sequence from Drosophila melanogaster. Full crystallographic information is available from OCA.
Reference
The crystal structure of the minus-end-directed microtubule motor protein ncd reveals variable dimer conformations., Kozielski F, De Bonis S, Burmeister WP, Cohen-Addad C, Wade RH, Structure. 1999 Nov 15;7(11):1407-16. PMID:10574799
Page seeded by OCA on Thu Mar 20 10:30:40 2008
