1opy
From Proteopedia
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[[Category: pseudomonas putida biotype b]] | [[Category: pseudomonas putida biotype b]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 15:58:19 2007'' |
Revision as of 13:53, 30 October 2007
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KSI
Overview
The three-dimensional structure of the enzyme 3-oxo-delta5-steroid, isomerase (E.C. 5.3.3.1), a 28-kilodalton symmetrical dimer, was solved by, multidimensional heteronuclear magnetic resonance spectroscopy. The two, independently folded monomers pack together by means of extensive, hydrophobic and electrostatic interactions. Each monomer comprises three, alpha helices and a six-strand mixed beta-pleated sheet arranged to form a, deep hydrophobic cavity. Catalytically important residues Tyr14 (general, acid) and Asp38 (general base) are located near the bottom of the cavity, and positioned as expected from mechanistic hypotheses. An unexpected acid, group (Asp99) is also located in the active site adjacent to Tyr14, and, kinetic and binding studies of the Asp99 to Ala mutant demonstrate ... [(full description)]
About this Structure
1OPY is a [Single protein] structure of sequence from [Pseudomonas putida]. Active as [Steroid Delta-isomerase], with EC number [5.3.3.1]. Structure known Active Site: A. Full crystallographic information is available from [OCA].
Reference
Solution structure of 3-oxo-delta5-steroid isomerase., Wu ZR, Ebrahimian S, Zawrotny ME, Thornburg LD, Perez-Alvarado GC, Brothers P, Pollack RM, Summers MF, Science. 1997 Apr 18;276(5311):415-8. PMID:9103200
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