1d3v
From Proteopedia
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| - | [[Image:1d3v.gif|left|200px]] | + | [[Image:1d3v.gif|left|200px]] |
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| - | '''CRYSTAL STRUCTURE OF THE BINUCLEAR MANGANESE METALLOENZYME ARGINASE COMPLEXED WITH 2(S)-AMINO-6-BORONOHEXANOIC ACID, AN L-ARGININE ANALOG''' | + | {{Structure |
| + | |PDB= 1d3v |SIZE=350|CAPTION= <scene name='initialview01'>1d3v</scene>, resolution 1.70Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene> and <scene name='pdbligand=ABH:2(S)-AMINO-6-BORONOHEXANOIC ACID'>ABH</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Arginase Arginase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.3.1 3.5.3.1] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''CRYSTAL STRUCTURE OF THE BINUCLEAR MANGANESE METALLOENZYME ARGINASE COMPLEXED WITH 2(S)-AMINO-6-BORONOHEXANOIC ACID, AN L-ARGININE ANALOG''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1D3V is a [ | + | 1D3V is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D3V OCA]. |
==Reference== | ==Reference== | ||
| - | Arginase-boronic acid complex highlights a physiological role in erectile function., Cox JD, Kim NN, Traish AM, Christianson DW, Nat Struct Biol. 1999 Nov;6(11):1043-7. PMID:[http:// | + | Arginase-boronic acid complex highlights a physiological role in erectile function., Cox JD, Kim NN, Traish AM, Christianson DW, Nat Struct Biol. 1999 Nov;6(11):1043-7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10542097 10542097] |
[[Category: Arginase]] | [[Category: Arginase]] | ||
[[Category: Rattus norvegicus]] | [[Category: Rattus norvegicus]] | ||
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[[Category: perfectly twinned crystal]] | [[Category: perfectly twinned crystal]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:32:30 2008'' |
Revision as of 08:32, 20 March 2008
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| , resolution 1.70Å | |||||||
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| Ligands: | and | ||||||
| Activity: | Arginase, with EC number 3.5.3.1 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
CRYSTAL STRUCTURE OF THE BINUCLEAR MANGANESE METALLOENZYME ARGINASE COMPLEXED WITH 2(S)-AMINO-6-BORONOHEXANOIC ACID, AN L-ARGININE ANALOG
Overview
The crystal structure of the complex between the binuclear manganese metalloenzyme arginase and the boronic acid analog of L-arginine, 2(S)-amino-6-boronohexanoic acid (ABH), has been determined at 1.7 A resolution from a crystal perfectly twinned by hemihedry. ABH binds as the tetrahedral boronate anion, with one hydroxyl oxygen symmetrically bridging the binuclear manganese cluster and a second hydroxyl oxygen coordinating to Mn2+A. This binding mode mimics the transition state of a metal-activated hydroxide mechanism. This transition state structure differs from that occurring in NO biosynthesis, thereby explaining why ABH does not inhibit NO synthase. We also show that arginase activity is present in the penis. Accordingly, the tight binding and specificity of ABH allows us to probe the physiological role of arginase in modulating the NO-dependent smooth muscle relaxation required for erection. Strikingly, ABH causes significant enhancement of nonadrenergic, noncholinergic nerve-mediated relaxation of penile corpus cavernosum smooth muscle, suggesting that arginase inhibition sustains L-arginine concentrations for NO synthase activity. Therefore, human penile arginase is a potential target for therapeutic intervention in the treatment of erectile dysfunction.
About this Structure
1D3V is a Single protein structure of sequence from Rattus norvegicus. Full crystallographic information is available from OCA.
Reference
Arginase-boronic acid complex highlights a physiological role in erectile function., Cox JD, Kim NN, Traish AM, Christianson DW, Nat Struct Biol. 1999 Nov;6(11):1043-7. PMID:10542097
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