1d8h

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[[Image:1d8h.gif|left|200px]]<br /><applet load="1d8h" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1d8h.gif|left|200px]]
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caption="1d8h, resolution 2.00&Aring;" />
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'''X-RAY CRYSTAL STRUCTURE OF YEAST RNA TRIPHOSPHATASE IN COMPLEX WITH SULFATE AND MANGANESE IONS.'''<br />
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{{Structure
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|PDB= 1d8h |SIZE=350|CAPTION= <scene name='initialview01'>1d8h</scene>, resolution 2.00&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene> and <scene name='pdbligand=SO4:SULFATE ION'>SO4</scene>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Polynucleotide_5'-phosphatase Polynucleotide 5'-phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.33 3.1.3.33]
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|GENE=
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}}
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'''X-RAY CRYSTAL STRUCTURE OF YEAST RNA TRIPHOSPHATASE IN COMPLEX WITH SULFATE AND MANGANESE IONS.'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1D8H is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with <scene name='pdbligand=MN:'>MN</scene> and <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Polynucleotide_5'-phosphatase Polynucleotide 5'-phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.33 3.1.3.33] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D8H OCA].
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1D8H is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D8H OCA].
==Reference==
==Reference==
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Structure and mechanism of yeast RNA triphosphatase: an essential component of the mRNA capping apparatus., Lima CD, Wang LK, Shuman S, Cell. 1999 Nov 24;99(5):533-43. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10589681 10589681]
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Structure and mechanism of yeast RNA triphosphatase: an essential component of the mRNA capping apparatus., Lima CD, Wang LK, Shuman S, Cell. 1999 Nov 24;99(5):533-43. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10589681 10589681]
[[Category: Polynucleotide 5'-phosphatase]]
[[Category: Polynucleotide 5'-phosphatase]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
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[[Category: rna triphosphatase]]
[[Category: rna triphosphatase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:14:04 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:34:30 2008''

Revision as of 08:34, 20 March 2008

Image:1d8h.gif


PDB ID 1d8h

Drag the structure with the mouse to rotate
, resolution 2.00Å
Ligands: and
Activity: Polynucleotide 5'-phosphatase, with EC number 3.1.3.33
Coordinates: save as pdb, mmCIF, xml



X-RAY CRYSTAL STRUCTURE OF YEAST RNA TRIPHOSPHATASE IN COMPLEX WITH SULFATE AND MANGANESE IONS.


Overview

RNA triphosphatase is an essential mRNA processing enzyme that catalyzes the first step in cap formation. The 2.05 A crystal structure of yeast RNA triphosphatase Cet1p reveals a novel active site fold whereby an eight-stranded beta barrel forms a topologically closed triphosphate tunnel. Interactions of a sulfate in the center of the tunnel with a divalent cation and basic amino acids projecting into the tunnel suggest a catalytic mechanism that is supported by mutational data. Discrete surface domains mediate Cet1p homodimerization and Cet1p binding to the guanylyltransferase component of the capping apparatus. The structure and mechanism of fungal RNA triphosphatases are completely different from those of mammalian mRNA capping enzymes. Hence, RNA triphosphatase presents an ideal target for structure-based antifungal drug discovery.

About this Structure

1D8H is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.

Reference

Structure and mechanism of yeast RNA triphosphatase: an essential component of the mRNA capping apparatus., Lima CD, Wang LK, Shuman S, Cell. 1999 Nov 24;99(5):533-43. PMID:10589681

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