1dgo
From Proteopedia
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| - | [[Image:1dgo.gif|left|200px]] | + | [[Image:1dgo.gif|left|200px]] |
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| - | '''SOLUTION STRUCTURE OF A URACIL CONTAINING HAIRPIN DNA''' | + | {{Structure |
| + | |PDB= 1dgo |SIZE=350|CAPTION= <scene name='initialview01'>1dgo</scene> | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
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| + | '''SOLUTION STRUCTURE OF A URACIL CONTAINING HAIRPIN DNA''' | ||
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==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1DGO is a [ | + | 1DGO is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DGO OCA]. |
==Reference== | ==Reference== | ||
| - | Structural basis for uracil DNA glycosylase interaction with uracil: NMR study., Ghosh M, Vinay Kumar N, Varshney U, Chary KV, Nucleic Acids Res. 2000 May 1;28(9):1906-12. PMID:[http:// | + | Structural basis for uracil DNA glycosylase interaction with uracil: NMR study., Ghosh M, Vinay Kumar N, Varshney U, Chary KV, Nucleic Acids Res. 2000 May 1;28(9):1906-12. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10756190 10756190] |
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Chary, K V.R.]] | [[Category: Chary, K V.R.]] | ||
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[[Category: uracil]] | [[Category: uracil]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:38:17 2008'' |
Revision as of 08:38, 20 March 2008
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SOLUTION STRUCTURE OF A URACIL CONTAINING HAIRPIN DNA
Overview
Two dimensional (2D) NMR and molecular dynamics simulations have been used to determine the three dimensional (3D) structure of a hairpin DNA, d-CTA-GAGGATCC-TUTT-GGATCCT (22mer; abbreviated as U2-hairpin), which has uracil at the second position from the 5' end of the tetraloop. The(1)H resonances of this hairpin have been assigned almost completely. NMR restrained molecular dynamics and energy minimization procedures have been used to describe the 3D structure of U2-hairpin. This study establishes that the stem of the hairpin adopts a right-handed B-DNA conformation, while the T(12)and T(15)nucleotides stack upon 3' and 5' ends of the stem, respectively. Further, T(14)stacks upon both T(12)and T(15). Though U(13)partially stacks upon T(14), no stacking interaction is observed between U(13)and T(12). All the individual nucleotide bases belonging to the stem and T(12)and T(15)of the loop adopt ' anti ' conformation with respect to their sugar moiety, while the U(13)and T(14)of the loop are in ' syn ' conformation. The turning phosphate in the loop is located between T(13)and T(14). This study and a concurrent NMR structural study on yet another hairpin DNA d-CTAGAGGAATAA-TTTU-GGATCCT (22mer; abbreviated as U4-hairpin), with uracil at the fourth position from the 5' end of the tetraloop throw light upon various interactions which have been reported between Escherichia coli uracil DNA glycosylase (UDG) and uracil containing DNA. The epsilon of T(12)and alpha, beta, gamma, epsilon and zeta of U(13)and gamma of T(14), which partially influence the local conformation of U(13)in U2-hairpin are all locked in ' trans ' conformation. Such stretched out backbone conformation in the vicinity of U(13)could be the reason as to why the U2-hairpin is found to be the poor substrate for its interaction with UDG compared to the other substrates in which the uracil is at first, third and fourth positions of the tetraloop from its 5' end, as reported earlier by Vinay and Varshney. This study shows that UDG actively promotes the flipping of uracil from a stacked conformation and rules out the possibility of UDG recognizing the flipped out uracil bases.
About this Structure
1DGO is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Structural basis for uracil DNA glycosylase interaction with uracil: NMR study., Ghosh M, Vinay Kumar N, Varshney U, Chary KV, Nucleic Acids Res. 2000 May 1;28(9):1906-12. PMID:10756190
Page seeded by OCA on Thu Mar 20 10:38:17 2008
