1ogv

From Proteopedia

Revision as of 13:49, 30 October 2007

LIPIDIC CUBIC PHASE CRYSTAL STRUCTURE OF THE PHOTOSYNTHETIC REACTION CENTRE FROM RHODOBACTER SPHAEROIDES

Overview

Well-ordered crystals of the bacterial photosynthetic reaction centre from, Rhodobacter sphaeroides were grown from a lipidic cubic phase. Here, we, report the type I crystal packing that results from this crystallisation, medium, for which 3D crystals grow as stacked 2D crystals, and the, reaction centre X-ray structure is refined to 2.35A resolution. In this, crystal form, the location of the membrane bilayer could be assigned with, confidence. A cardiolipin-binding site is found at the protein-protein, interface within the membrane-spanning region, shedding light on the, formation of crystal contacts within the membrane. A chloride-binding site, was identified in the membrane-spanning region, which suggests a putative, site for interaction with the light-harvesting complex I, the ... [(full description)]

About this Structure

1OGV is a [Protein complex] structure of sequences from [Rhodobacter sphaeroides] with FE2, CL, BCL, BPH, U10 and CDL as [ligands]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].

Reference

Lipidic cubic phase crystal structure of the photosynthetic reaction centre from Rhodobacter sphaeroides at 2.35A resolution., Katona G, Andreasson U, Landau EM, Andreasson LE, Neutze R, J Mol Biol. 2003 Aug 15;331(3):681-92. PMID:12899837

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