1e5i
From Proteopedia
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| - | [[Image:1e5i.jpg|left|200px]] | + | [[Image:1e5i.jpg|left|200px]] |
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| - | '''DELTA-R306 DEACETOXYCEPHALOSPORIN C SYNTHASE COMPLEXED WITH IRON AND 2-OXOGLUTARATE.''' | + | {{Structure |
| + | |PDB= 1e5i |SIZE=350|CAPTION= <scene name='initialview01'>1e5i</scene>, resolution 2.10Å | ||
| + | |SITE= <scene name='pdbsite=AKG:Alpha-Ketoglutarate+Binding+Site'>AKG</scene> and <scene name='pdbsite=FE:Fe+Site+To+Which+Non-Protein+Ligand+Is+Attached+To+Fe'>FE</scene> | ||
| + | |LIGAND= <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene> and <scene name='pdbligand=AKG:2-OXYGLUTARIC ACID'>AKG</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= CEFE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1901 Streptomyces clavuligerus]) | ||
| + | }} | ||
| + | |||
| + | '''DELTA-R306 DEACETOXYCEPHALOSPORIN C SYNTHASE COMPLEXED WITH IRON AND 2-OXOGLUTARATE.''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1E5I is a [ | + | 1E5I is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_clavuligerus Streptomyces clavuligerus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E5I OCA]. |
==Reference== | ==Reference== | ||
| - | Kinetic and crystallographic studies on deacetoxycephalosporin C synthase (DAOCS)., Lee HJ, Lloyd MD, Harlos K, Clifton IJ, Baldwin JE, Schofield CJ, J Mol Biol. 2001 May 18;308(5):937-48. PMID:[http:// | + | Kinetic and crystallographic studies on deacetoxycephalosporin C synthase (DAOCS)., Lee HJ, Lloyd MD, Harlos K, Clifton IJ, Baldwin JE, Schofield CJ, J Mol Biol. 2001 May 18;308(5):937-48. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11352583 11352583] |
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Streptomyces clavuligerus]] | [[Category: Streptomyces clavuligerus]] | ||
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[[Category: FE2]] | [[Category: FE2]] | ||
[[Category: 2-oxoglutarate]] | [[Category: 2-oxoglutarate]] | ||
| - | [[Category: c-terminus | + | [[Category: c-terminus antibiotic]] |
[[Category: cephalosporin]] | [[Category: cephalosporin]] | ||
[[Category: ferrous oxygenase]] | [[Category: ferrous oxygenase]] | ||
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[[Category: oxidoreductase]] | [[Category: oxidoreductase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:50:00 2008'' |
Revision as of 08:50, 20 March 2008
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| , resolution 2.10Å | |||||||
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| Sites: | and | ||||||
| Ligands: | and | ||||||
| Gene: | CEFE (Streptomyces clavuligerus) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
DELTA-R306 DEACETOXYCEPHALOSPORIN C SYNTHASE COMPLEXED WITH IRON AND 2-OXOGLUTARATE.
Overview
Deacetoxycephalosporin C synthase (DAOCS) is an iron(II) and 2-oxoglutarate-dependent oxygenase that catalyzes the conversion of penicillin N to deacetoxycephalosporin C, the committed step in the biosynthesis of cephalosporin antibiotics. The crystal structure of DAOCS revealed that the C terminus of one molecule is inserted into the active site of its neighbor in a cyclical fashion within a trimeric unit. This arrangement has hindered the generation of crystalline enzyme-substrate complexes. Therefore, we constructed a series of DAOCS mutants with modified C termini. Oxidation of 2-oxoglutarate was significantly uncoupled from oxidation of the penicillin substrate in certain truncated mutants. The extent of uncoupling varied with the number of residues deleted and the penicillin substrate used. Crystal structures were determined for the DeltaR306 mutant complexed with iron(II) and 2-oxoglutarate (to 2.10 A) and the DeltaR306A mutant complexed with iron(II), succinate and unhydrated carbon dioxide (to 1.96 A). The latter may mimic a product complex, and supports proposals for a metal-bound CO(2) intermediate during catalysis.
About this Structure
1E5I is a Single protein structure of sequence from Streptomyces clavuligerus. Full crystallographic information is available from OCA.
Reference
Kinetic and crystallographic studies on deacetoxycephalosporin C synthase (DAOCS)., Lee HJ, Lloyd MD, Harlos K, Clifton IJ, Baldwin JE, Schofield CJ, J Mol Biol. 2001 May 18;308(5):937-48. PMID:11352583
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