1efn

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[[Image:1efn.gif|left|200px]]<br /><applet load="1efn" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1efn.gif|left|200px]]
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caption="1efn, resolution 2.5&Aring;" />
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'''HIV-1 NEF PROTEIN IN COMPLEX WITH R96I MUTANT FYN SH3 DOMAIN'''<br />
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{{Structure
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|PDB= 1efn |SIZE=350|CAPTION= <scene name='initialview01'>1efn</scene>, resolution 2.5&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=PBM:TRIMETHYL LEAD ION'>PBM</scene>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Transferase Transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.10.1 and 2.7.10.2 2.7.10.1 and 2.7.10.2]
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|GENE= HIV-1 NEF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]), HIV-1 NEF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11676 Human immunodeficiency virus 1])
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}}
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'''HIV-1 NEF PROTEIN IN COMPLEX WITH R96I MUTANT FYN SH3 DOMAIN'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1EFN is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Human_immunodeficiency_virus_1 Human immunodeficiency virus 1] with <scene name='pdbligand=PBM:'>PBM</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Transferase Transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.10.1 and 2.7.10.2 2.7.10.1 and 2.7.10.2] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EFN OCA].
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1EFN is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Human_immunodeficiency_virus_1 Human immunodeficiency virus 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EFN OCA].
==Reference==
==Reference==
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Crystal structure of the conserved core of HIV-1 Nef complexed with a Src family SH3 domain., Lee CH, Saksela K, Mirza UA, Chait BT, Kuriyan J, Cell. 1996 Jun 14;85(6):931-42. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8681387 8681387]
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Crystal structure of the conserved core of HIV-1 Nef complexed with a Src family SH3 domain., Lee CH, Saksela K, Mirza UA, Chait BT, Kuriyan J, Cell. 1996 Jun 14;85(6):931-42. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8681387 8681387]
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Human immunodeficiency virus 1]]
[[Category: Human immunodeficiency virus 1]]
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[[Category: Lee, C H.]]
[[Category: Lee, C H.]]
[[Category: PBM]]
[[Category: PBM]]
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[[Category: aids]]
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[[Category: aid]]
[[Category: atp-binding]]
[[Category: atp-binding]]
[[Category: complex (sh3 domain/viral enhancer)]]
[[Category: complex (sh3 domain/viral enhancer)]]
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[[Category: tyrosine-protein kinase]]
[[Category: tyrosine-protein kinase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:27:14 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:54:55 2008''

Revision as of 08:54, 20 March 2008

Image:1efn.gif


PDB ID 1efn

Drag the structure with the mouse to rotate
, resolution 2.5Å
Ligands:
Gene: HIV-1 NEF (Homo sapiens), HIV-1 NEF (Human immunodeficiency virus 1)
Activity: Transferase, with EC number and 2.7.10.2 2.7.10.1 and 2.7.10.2
Coordinates: save as pdb, mmCIF, xml



HIV-1 NEF PROTEIN IN COMPLEX WITH R96I MUTANT FYN SH3 DOMAIN


Overview

The crystal structure of the conserved core of HIV-1 Nef has been determined in complex with the SH3 domain of a mutant Fyn tyrosine kinase (a single amino acid substitution, Arg-96 to isoleucine), to which Nef binds tightly. The conserved PxxP sequence motif of Nef, known to be important for optimal viral replication, is part of a polyproline type II helix that engages the SH3 domain in a manner resembling closely the interaction of isolated peptides with SH3 domains. The Nef-SH3 structure also reveals how high affinity and specificity in the SH3 interaction is achieved by the presentation of the PxxP motif within the context of the folded structure of Nef.

About this Structure

1EFN is a Protein complex structure of sequences from Homo sapiens and Human immunodeficiency virus 1. Full crystallographic information is available from OCA.

Reference

Crystal structure of the conserved core of HIV-1 Nef complexed with a Src family SH3 domain., Lee CH, Saksela K, Mirza UA, Chait BT, Kuriyan J, Cell. 1996 Jun 14;85(6):931-42. PMID:8681387

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