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1efx

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[[Image:1efx.jpg|left|200px]]<br /><applet load="1efx" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1efx.jpg|left|200px]]
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caption="1efx, resolution 3.0&Aring;" />
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'''STRUCTURE OF A COMPLEX BETWEEN THE HUMAN NATURAL KILLER CELL RECEPTOR KIR2DL2 AND A CLASS I MHC LIGAND HLA-CW3'''<br />
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{{Structure
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|PDB= 1efx |SIZE=350|CAPTION= <scene name='initialview01'>1efx</scene>, resolution 3.0&Aring;
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|SITE=
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|LIGAND=
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|ACTIVITY=
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'''STRUCTURE OF A COMPLEX BETWEEN THE HUMAN NATURAL KILLER CELL RECEPTOR KIR2DL2 AND A CLASS I MHC LIGAND HLA-CW3'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1EFX is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EFX OCA].
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1EFX is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EFX OCA].
==Reference==
==Reference==
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Crystal structure of an NK cell immunoglobulin-like receptor in complex with its class I MHC ligand., Boyington JC, Motyka SA, Schuck P, Brooks AG, Sun PD, Nature. 2000 Jun 1;405(6786):537-43. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10850706 10850706]
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Crystal structure of an NK cell immunoglobulin-like receptor in complex with its class I MHC ligand., Boyington JC, Motyka SA, Schuck P, Brooks AG, Sun PD, Nature. 2000 Jun 1;405(6786):537-43. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10850706 10850706]
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Protein complex]]
[[Category: Protein complex]]
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[[Category: receptor/mhc complex]]
[[Category: receptor/mhc complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:27:18 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:55:05 2008''

Revision as of 08:55, 20 March 2008


PDB ID 1efx

Drag the structure with the mouse to rotate
, resolution 3.0Å
Coordinates: save as pdb, mmCIF, xml



STRUCTURE OF A COMPLEX BETWEEN THE HUMAN NATURAL KILLER CELL RECEPTOR KIR2DL2 AND A CLASS I MHC LIGAND HLA-CW3


Contents

Overview

Target cell lysis is regulated by natural killer (NK) cell receptors that recognize class I MHC molecules. Here we report the crystal structure of the human immunoglobulin-like NK cell receptor KIR2DL2 in complex with its class I ligand HLA-Cw3 and peptide. KIR binds in a nearly orthogonal orientation across the alpha1 and alpha2 helices of Cw3 and directly contacts positions 7 and 8 of the peptide. No significant conformational changes in KIR occur on complex formation. The receptor footprint on HLA overlaps with but is distinct from that of the T-cell receptor. Charge complementarity dominates the KIR/HLA interface and mutations that disrupt interface salt bridges substantially diminish binding. Most contacts in the complex are between KIR and conserved HLA-C residues, but a hydrogen bond between Lys 44 of KIR2DL2 and Asn 80 of Cw3 confers the allotype specificity. KIR contact requires position 8 of the peptide to be a residue smaller than valine. A second KIR/HLA interface produced an ordered receptor-ligand aggregation in the crystal which may resemble receptor clustering during immune synapse formation.

Disease

Known disease associated with this structure: Hypoproteinemia, hypercatabolic OMIM:[109700]

About this Structure

1EFX is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Crystal structure of an NK cell immunoglobulin-like receptor in complex with its class I MHC ligand., Boyington JC, Motyka SA, Schuck P, Brooks AG, Sun PD, Nature. 2000 Jun 1;405(6786):537-43. PMID:10850706

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