1eja
From Proteopedia
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| - | [[Image:1eja.gif|left|200px]] | + | [[Image:1eja.gif|left|200px]] |
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| - | '''STRUCTURE OF PORCINE TRYPSIN COMPLEXED WITH BDELLASTASIN, AN ANTISTASIN-TYPE INHIBITOR''' | + | {{Structure |
| + | |PDB= 1eja |SIZE=350|CAPTION= <scene name='initialview01'>1eja</scene>, resolution 2.7Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=NA:SODIUM ION'>NA</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''STRUCTURE OF PORCINE TRYPSIN COMPLEXED WITH BDELLASTASIN, AN ANTISTASIN-TYPE INHIBITOR''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1EJA is a [ | + | 1EJA is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Hirudo_medicinalis Hirudo medicinalis] and [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EJA OCA]. |
==Reference== | ==Reference== | ||
| - | L-Isoaspartate 115 of porcine beta-trypsin promotes crystallization of its complex with bdellastasin., Rester U, Moser M, Huber R, Bode W, Acta Crystallogr D Biol Crystallogr. 2000 May;56(Pt 5):581-8. PMID:[http:// | + | L-Isoaspartate 115 of porcine beta-trypsin promotes crystallization of its complex with bdellastasin., Rester U, Moser M, Huber R, Bode W, Acta Crystallogr D Biol Crystallogr. 2000 May;56(Pt 5):581-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10771427 10771427] |
[[Category: Hirudo medicinalis]] | [[Category: Hirudo medicinalis]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
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[[Category: trypsin]] | [[Category: trypsin]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:56:21 2008'' |
Revision as of 08:56, 20 March 2008
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| , resolution 2.7Å | |||||||
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| Ligands: | |||||||
| Activity: | Trypsin, with EC number 3.4.21.4 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
STRUCTURE OF PORCINE TRYPSIN COMPLEXED WITH BDELLASTASIN, AN ANTISTASIN-TYPE INHIBITOR
Overview
Bdellastasin is a 59-amino-acid, cysteine-rich, antistasin-type inhibitor of sperm acrosin, plasmin and trypsin, isolated from the medicinal leech Hirudo medicinalis. The complex formed between bdellastasin and porcine beta-trypsin has previously been crystallized in the presence of PEG in a tetragonal crystal form of space group P4(3)2(1)2 and has now been found to crystallize under high-salt conditions in the enantiomorphic space group P4(1)2(1)2. These structures have been solved and refined to 2.8 and 2.7 A resolution, respectively. Bdellastasin turns out to have an antistasin-like fold exhibiting a bis-domainal structure. In the second new crystal form, the flexible N-terminal subdomain is rotated with respect to the C--terminal subdomain by about 90 degrees, fitting into a cavity formed by symmetry-related trypsin molecules. The canonical inhibitor-proteinase interaction is restricted to the primary binding loop comprising residues Leu31-Lys36 of bdellastasin. During the refinement, a bound sodium ion occupying the calcium-binding site of the porcine beta-trypsin component was discovered. This sodium ion is coordinated in a tetragonal-pyramidal manner, with the geometry of the enclosing loop slightly changed compared with the loop in the presence of calcium. In the crystal form of space group P4(3)2(1)2, the electron density for residue 115 of porcine beta-trypsin clearly indicates the presence of a beta-isomerized L-aspartic acid, which is placed in spatial proximity to segment Thr144--Gly148 of a symmetry-related trypsin molecule. This is the first structurally observed example of an L-isoaspartate in beta--trypsin originating from Asn. A comparison with other known crystal structures of porcine beta-trypsin-macromolecular inhibitor complexes suggests that the deamidation, isomerization and racemization of Asn115 is the key step in crystallization.
About this Structure
1EJA is a Protein complex structure of sequences from Hirudo medicinalis and Sus scrofa. Full crystallographic information is available from OCA.
Reference
L-Isoaspartate 115 of porcine beta-trypsin promotes crystallization of its complex with bdellastasin., Rester U, Moser M, Huber R, Bode W, Acta Crystallogr D Biol Crystallogr. 2000 May;56(Pt 5):581-8. PMID:10771427
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