1elk
From Proteopedia
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| - | [[Image:1elk.gif|left|200px]] | + | [[Image:1elk.gif|left|200px]] |
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| - | '''VHS domain of TOM1 protein from H. sapiens''' | + | {{Structure |
| + | |PDB= 1elk |SIZE=350|CAPTION= <scene name='initialview01'>1elk</scene>, resolution 1.5Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
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| + | '''VHS domain of TOM1 protein from H. sapiens''' | ||
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==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1ELK is a [ | + | 1ELK is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ELK OCA]. |
==Reference== | ==Reference== | ||
| - | Structure of the VHS domain of human Tom1 (target of myb 1): insights into interactions with proteins and membranes., Misra S, Beach BM, Hurley JH, Biochemistry. 2000 Sep 19;39(37):11282-90. PMID:[http:// | + | Structure of the VHS domain of human Tom1 (target of myb 1): insights into interactions with proteins and membranes., Misra S, Beach BM, Hurley JH, Biochemistry. 2000 Sep 19;39(37):11282-90. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10985773 10985773] |
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: Hurley, J H.]] | [[Category: Hurley, J H.]] | ||
[[Category: Misra, S.]] | [[Category: Misra, S.]] | ||
| - | [[Category: superhelix of | + | [[Category: superhelix of helice]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:57:16 2008'' |
Revision as of 08:57, 20 March 2008
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| Coordinates: | save as pdb, mmCIF, xml | ||||||
VHS domain of TOM1 protein from H. sapiens
Overview
VHS domains are found at the N-termini of select proteins involved in intracellular membrane trafficking. We have determined the crystal structure of the VHS domain of the human Tom1 (target of myb 1) protein to 1.5 A resolution. The domain consists of eight helices arranged in a superhelix. The surface of the domain has two main features: (1) a basic patch on one side due to several conserved positively charged residues on helix 3 and (2) a negatively charged ridge on the opposite side, formed by residues on helix 2. We compare our structure to the recently obtained structure of tandem VHS-FYVE domains from Hrs [Mao, Y., Nickitenko, A., Duan, X., Lloyd, T. E., Wu, M. N., Bellen, H., and Quiocho, F. A. (2000) Cell 100, 447-456]. Key features of the interaction surface between the FYVE and VHS domains of Hrs, involving helices 2 and 4 of the VHS domain, are conserved in the VHS domain of Tom1, even though Tom1 does not have a FYVE domain. We also compare the structures of the VHS domains of Tom1 and Hrs to the recently obtained structure of the ENTH domain of epsin-1 [Hyman, J., Chen, H., Di Fiore, P. P., De Camilli, P., and Brunger, A. T. (2000) J. Cell Biol. 149, 537-546]. Comparison of the two VHS domains and the ENTH domain reveals a conserved surface, composed of helices 2 and 4, that is utilized for protein-protein interactions. In addition, VHS domain-containing proteins are often localized to membranes. We suggest that the conserved positively charged surface of helix 3 in VHS and ENTH domains plays a role in membrane binding.
About this Structure
1ELK is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Structure of the VHS domain of human Tom1 (target of myb 1): insights into interactions with proteins and membranes., Misra S, Beach BM, Hurley JH, Biochemistry. 2000 Sep 19;39(37):11282-90. PMID:10985773
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