1eq9
From Proteopedia
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| - | [[Image:1eq9.gif|left|200px]] | + | [[Image:1eq9.gif|left|200px]] |
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| - | '''CRYSTAL STRUCTURE OF FIRE ANT CHYMOTRYPSIN COMPLEXED TO PMSF''' | + | {{Structure |
| + | |PDB= 1eq9 |SIZE=350|CAPTION= <scene name='initialview01'>1eq9</scene>, resolution 1.70Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=PMS:BENZYLSULFINIC ACID'>PMS</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Chymotrypsin Chymotrypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.1 3.4.21.1] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''CRYSTAL STRUCTURE OF FIRE ANT CHYMOTRYPSIN COMPLEXED TO PMSF''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1EQ9 is a [ | + | 1EQ9 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Solenopsis_invicta Solenopsis invicta]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EQ9 OCA]. |
==Reference== | ==Reference== | ||
| - | The structure of an insect chymotrypsin., Botos I, Meyer E, Nguyen M, Swanson SM, Koomen JM, Russell DH, Meyer EF, J Mol Biol. 2000 May 19;298(5):895-901. PMID:[http:// | + | The structure of an insect chymotrypsin., Botos I, Meyer E, Nguyen M, Swanson SM, Koomen JM, Russell DH, Meyer EF, J Mol Biol. 2000 May 19;298(5):895-901. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10801356 10801356] |
[[Category: Chymotrypsin]] | [[Category: Chymotrypsin]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: serine proteinase]] | [[Category: serine proteinase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:58:58 2008'' |
Revision as of 08:58, 20 March 2008
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| , resolution 1.70Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Activity: | Chymotrypsin, with EC number 3.4.21.1 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
CRYSTAL STRUCTURE OF FIRE ANT CHYMOTRYPSIN COMPLEXED TO PMSF
Overview
The South American imported fire ant (Solenopsis invicta), without natural enemies in the United States, widely infests the southern United States, causing more than a half billion dollars in health and agriculture-related damage annually in Texas alone. Fire ants are resistant to most insecticides, so control will require a more fundamental understanding of their biochemistry and metabolism leading to the design of selective, ecologically safe insecticides. The 4th instar larvae play a crucial role in the nutrition of the colony by secreting proteinases (especially chymotrypsin) which digest food products for the entire colony. The first structure of an ant proteolytic enzyme, fire ant chymotrypsin, was determined to atomic resolution (1.7 A). A structural comparison of the ant and mammalian structures confirms the "universality" of the serine proteinase motif and reveals a difference at residues 147-148, which are proteolytically removed in the bovine enzyme but are firmly intact in the ant chymotrypsin, suggesting a different activation mechanism for the latter. Likewise, the absence of the covalently attached propeptide domain (1-15) further suggests an uncharacteristic activation mechanism. The presence of Gly189 in the S1 site is an atypical feature of this chymotrypsin and is comparable only to human leukocyte elastase, hornet chymotrypsin and fiddler crab collagenase. Binding studies confirm the chymotrypsin nature of this novel enzyme.
About this Structure
1EQ9 is a Single protein structure of sequence from Solenopsis invicta. Full crystallographic information is available from OCA.
Reference
The structure of an insect chymotrypsin., Botos I, Meyer E, Nguyen M, Swanson SM, Koomen JM, Russell DH, Meyer EF, J Mol Biol. 2000 May 19;298(5):895-901. PMID:10801356
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