1eqn
From Proteopedia
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| - | [[Image:1eqn.gif|left|200px]] | + | [[Image:1eqn.gif|left|200px]] |
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| - | '''E.COLI PRIMASE CATALYTIC CORE''' | + | {{Structure |
| + | |PDB= 1eqn |SIZE=350|CAPTION= <scene name='initialview01'>1eqn</scene>, resolution 2.9Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
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| + | '''E.COLI PRIMASE CATALYTIC CORE''' | ||
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==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1EQN is a [ | + | 1EQN is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EQN OCA]. |
==Reference== | ==Reference== | ||
| - | A TOPRIM domain in the crystal structure of the catalytic core of Escherichia coli primase confirms a structural link to DNA topoisomerases., Podobnik M, McInerney P, O'Donnell M, Kuriyan J, J Mol Biol. 2000 Jul 7;300(2):353-62. PMID:[http:// | + | A TOPRIM domain in the crystal structure of the catalytic core of Escherichia coli primase confirms a structural link to DNA topoisomerases., Podobnik M, McInerney P, O'Donnell M, Kuriyan J, J Mol Biol. 2000 Jul 7;300(2):353-62. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10873470 10873470] |
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: toprim domain]] | [[Category: toprim domain]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:59:06 2008'' |
Revision as of 08:59, 20 March 2008
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| Coordinates: | save as pdb, mmCIF, xml | ||||||
E.COLI PRIMASE CATALYTIC CORE
Overview
Primases synthesize short RNA strands on single-stranded DNA templates, thereby generating the hybrid duplexes required for the initiation of synthesis by DNA polymerases. We present the crystal structure of the catalytic unit of a primase enzyme, that of a approximately 320 residue fragment of Escherichia coli primase, determined at 2.9 A resolution. Central to the catalytic unit is a TOPRIM domain that is strikingly similar in its structure to that of corresponding domains in DNA topoisomerases, but is unrelated to the catalytic centers of other DNA or RNA polymerases. The catalytic domain of primase is crescent-shaped, and the concave face of the crescent is predicted to accommodate about 10 base-pairs of RNA-DNA duplex in a loose interaction, thereby limiting processivity.
About this Structure
1EQN is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
A TOPRIM domain in the crystal structure of the catalytic core of Escherichia coli primase confirms a structural link to DNA topoisomerases., Podobnik M, McInerney P, O'Donnell M, Kuriyan J, J Mol Biol. 2000 Jul 7;300(2):353-62. PMID:10873470
Page seeded by OCA on Thu Mar 20 10:59:06 2008
