1evm
From Proteopedia
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| - | [[Image:1evm.gif|left|200px]] | + | [[Image:1evm.gif|left|200px]] |
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| - | '''NMR OBSERVATION OF A-TETRAD''' | + | {{Structure |
| + | |PDB= 1evm |SIZE=350|CAPTION= <scene name='initialview01'>1evm</scene> | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
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| + | '''NMR OBSERVATION OF A-TETRAD''' | ||
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==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1EVM is a [ | + | 1EVM is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EVM OCA]. |
==Reference== | ==Reference== | ||
| - | NMR studies on truncated sequences of human telomeric DNA: observation of a novel A-tetrad., Patel PK, Koti AS, Hosur RV, Nucleic Acids Res. 1999 Oct 1;27(19):3836-43. PMID:[http:// | + | NMR studies on truncated sequences of human telomeric DNA: observation of a novel A-tetrad., Patel PK, Koti AS, Hosur RV, Nucleic Acids Res. 1999 Oct 1;27(19):3836-43. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10481022 10481022] |
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Hosur, R V.]] | [[Category: Hosur, R V.]] | ||
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[[Category: nmr structure]] | [[Category: nmr structure]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:00:50 2008'' |
Revision as of 09:00, 20 March 2008
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| Coordinates: | save as pdb, mmCIF, xml | ||||||
NMR OBSERVATION OF A-TETRAD
Overview
The structure of the telomeric DNA has been a subject of extensive investigation in recent years due to the realization that it has important functional roles to play in vivo and the observations that truncated telomeric sequences exhibit a great variety of 3D structures in aqueous solutions. In this context, we describe here NMR structural studies on two truncated human telomeric DNA sequences, d-AG(3)T and d-TAG(3)T in solutions containing K(+)ions. The G(3)stretches in both the oligonucleotides were seen to form parallel-stranded quadruplexes. However, the AG(3)segment as a whole, had different structural characteristics. The structure of d-AG(3)T revealed the formation of a novel A-tetrad, which was not seen in d-TAG(3)T. The A's in the tetrad had syn glycosidic conformation as opposed to the anti conformation of the G's in the G-tetrads. The A-tetrad stacked well over the adjacent G-tetrad and the twist angle at this step was smaller in d-AG(3)T than in d-TAG(3)T. These observations are expected to be significant from the point of view of structural diversity and recognition in telomeres.
About this Structure
1EVM is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
NMR studies on truncated sequences of human telomeric DNA: observation of a novel A-tetrad., Patel PK, Koti AS, Hosur RV, Nucleic Acids Res. 1999 Oct 1;27(19):3836-43. PMID:10481022
Page seeded by OCA on Thu Mar 20 11:00:50 2008
