1eyq

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[[Image:1eyq.jpg|left|200px]]<br /><applet load="1eyq" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1eyq.jpg|left|200px]]
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caption="1eyq, resolution 1.85&Aring;" />
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'''CHALCONE ISOMERASE AND NARINGENIN'''<br />
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{{Structure
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|PDB= 1eyq |SIZE=350|CAPTION= <scene name='initialview01'>1eyq</scene>, resolution 1.85&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> and <scene name='pdbligand=NAR:NARINGENIN'>NAR</scene>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Chalcone_isomerase Chalcone isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.5.1.6 5.5.1.6]
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|GENE=
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}}
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'''CHALCONE ISOMERASE AND NARINGENIN'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1EYQ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Medicago_sativa Medicago sativa] with <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=NAR:'>NAR</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Chalcone_isomerase Chalcone isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.5.1.6 5.5.1.6] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EYQ OCA].
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1EYQ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Medicago_sativa Medicago sativa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EYQ OCA].
==Reference==
==Reference==
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Structure and mechanism of the evolutionarily unique plant enzyme chalcone isomerase., Jez JM, Bowman ME, Dixon RA, Noel JP, Nat Struct Biol. 2000 Sep;7(9):786-91. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10966651 10966651]
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Structure and mechanism of the evolutionarily unique plant enzyme chalcone isomerase., Jez JM, Bowman ME, Dixon RA, Noel JP, Nat Struct Biol. 2000 Sep;7(9):786-91. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10966651 10966651]
[[Category: Chalcone isomerase]]
[[Category: Chalcone isomerase]]
[[Category: Medicago sativa]]
[[Category: Medicago sativa]]
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[[Category: flavonoid]]
[[Category: flavonoid]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:33:01 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:02:03 2008''

Revision as of 09:02, 20 March 2008

Image:1eyq.jpg


PDB ID 1eyq

Drag the structure with the mouse to rotate
, resolution 1.85Å
Ligands: and
Activity: Chalcone isomerase, with EC number 5.5.1.6
Coordinates: save as pdb, mmCIF, xml



CHALCONE ISOMERASE AND NARINGENIN


Overview

Chalcone isomerase (CHI) catalyzes the intramolecular cyclization of chalcone synthesized by chalcone synthase (CHS) into (2S)-naringenin, an essential compound in the biosynthesis of anthocyanin pigments, inducers of Rhizobium nodulation genes, and antimicrobial phytoalexins. The 1.85 A resolution crystal structure of alfalfa CHI in complex with (2S)-naringenin reveals a novel open-faced beta-sandwich fold. Currently, proteins with homologous primary sequences are found only in higher plants. The topology of the active site cleft defines the stereochemistry of the cyclization reaction. The structure and mutational analysis suggest a mechanism in which shape complementarity of the binding cleft locks the substrate into a constrained conformation that allows the reaction to proceed with a second-order rate constant approaching the diffusion controlled limit. This structure raises questions about the evolutionary history of this structurally unique plant enzyme.

About this Structure

1EYQ is a Single protein structure of sequence from Medicago sativa. Full crystallographic information is available from OCA.

Reference

Structure and mechanism of the evolutionarily unique plant enzyme chalcone isomerase., Jez JM, Bowman ME, Dixon RA, Noel JP, Nat Struct Biol. 2000 Sep;7(9):786-91. PMID:10966651

Page seeded by OCA on Thu Mar 20 11:02:03 2008

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