1ezv
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:1ezv.gif|left|200px]] | + | [[Image:1ezv.gif|left|200px]] |
| - | + | ||
| - | '''STRUCTURE OF THE YEAST CYTOCHROME BC1 COMPLEX CO-CRYSTALLIZED WITH AN ANTIBODY FV-FRAGMENT''' | + | {{Structure |
| + | |PDB= 1ezv |SIZE=350|CAPTION= <scene name='initialview01'>1ezv</scene>, resolution 2.3Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene>, <scene name='pdbligand=SMA:STIGMATELLIN+A'>SMA</scene> and <scene name='pdbligand=UQ6:5-(3,7,11,15,19,23-HEXAMETHYL-TETRACOSA-2,6,10,14,18,22-HEXAENYL)-2,3-DIMETHOXY-6-METHYL-BENZENE-1,4-DIOL'>UQ6</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Ubiquinol--cytochrome-c_reductase Ubiquinol--cytochrome-c reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.10.2.2 1.10.2.2] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''STRUCTURE OF THE YEAST CYTOCHROME BC1 COMPLEX CO-CRYSTALLIZED WITH AN ANTIBODY FV-FRAGMENT''' | ||
| + | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1EZV is a [ | + | 1EZV is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EZV OCA]. |
==Reference== | ==Reference== | ||
| - | Structure at 2.3 A resolution of the cytochrome bc(1) complex from the yeast Saccharomyces cerevisiae co-crystallized with an antibody Fv fragment., Hunte C, Koepke J, Lange C, Rossmanith T, Michel H, Structure. 2000 Jun 15;8(6):669-84. PMID:[http:// | + | Structure at 2.3 A resolution of the cytochrome bc(1) complex from the yeast Saccharomyces cerevisiae co-crystallized with an antibody Fv fragment., Hunte C, Koepke J, Lange C, Rossmanith T, Michel H, Structure. 2000 Jun 15;8(6):669-84. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10873857 10873857] |
[[Category: Mus musculus]] | [[Category: Mus musculus]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| Line 29: | Line 38: | ||
[[Category: cytochrome bc1 complex]] | [[Category: cytochrome bc1 complex]] | ||
[[Category: electron transfer]] | [[Category: electron transfer]] | ||
| - | [[Category: matrix processing | + | [[Category: matrix processing peptidase]] |
[[Category: mitochondria]] | [[Category: mitochondria]] | ||
[[Category: proton transfer]] | [[Category: proton transfer]] | ||
| Line 38: | Line 47: | ||
[[Category: yeast]] | [[Category: yeast]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:02:30 2008'' |
Revision as of 09:02, 20 March 2008
| |||||||
| , resolution 2.3Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , and | ||||||
| Activity: | Ubiquinol--cytochrome-c reductase, with EC number 1.10.2.2 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
STRUCTURE OF THE YEAST CYTOCHROME BC1 COMPLEX CO-CRYSTALLIZED WITH AN ANTIBODY FV-FRAGMENT
Overview
BACKGROUND: The cytochrome bc(1) complex is part of the energy conversion machinery of the respiratory and photosynthetic electron transfer chains. This integral membrane protein complex catalyzes electron transfer from ubiquinol to cytochrome c. It couples the electron transfer to the electrogenic translocation of protons across the membrane via a so-called Q cycle mechanism. RESULTS: The cytochrome bc(1) complex from the yeast Saccharomyces cerevisiae was crystallized together with a bound antibody Fv fragment. The structure was determined at 2.3 A resolution using multiple isomorphous replacement, and refined to a crystallographic R factor of 22.2% (R(free) = 25.4%). The complex is present as a homodimer. Each 'monomer' of the refined model includes 2178 amino acid residues of subunits COR1, QCR2, COB, CYT1, RIP1, QCR6, QCR7, QCR8 and QCR9 of the cytochrome bc(1) complex and of the polypeptides V(H) and V(L) of the Fv fragment, the cofactors heme b(H), heme b(L), heme c(1), the [2Fe-2S] cluster and 346 water molecules. The Fv fragment binds to the extrinsic domain of the [2Fe-2S] Rieske protein and is essential for formation of the crystal lattice. CONCLUSIONS: The approach to crystallize membrane proteins as complexes with specific antibody fragments appears to be of general importance. The structure of the yeast cytochrome bc(1) complex reveals in detail the binding sites of the natural substrate coenzyme Q6 and the inhibitor stigmatellin. Buried water molecules close to the binding sites suggest possible pathways for proton uptake and release. A comparison with other cytochrome bc(1) complexes shows features that are specific to yeast.
About this Structure
1EZV is a Protein complex structure of sequences from Mus musculus and Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
Reference
Structure at 2.3 A resolution of the cytochrome bc(1) complex from the yeast Saccharomyces cerevisiae co-crystallized with an antibody Fv fragment., Hunte C, Koepke J, Lange C, Rossmanith T, Michel H, Structure. 2000 Jun 15;8(6):669-84. PMID:10873857
Page seeded by OCA on Thu Mar 20 11:02:30 2008
Categories: Mus musculus | Protein complex | Saccharomyces cerevisiae | Ubiquinol--cytochrome-c reductase | Hunte, C. | Koepke, J. | Lange, C. | Michel, H. | Rossmanith, T. | FES | HEM | SMA | UQ6 | Antibody fv-fragment | Coenzyme q6 | Complex iii | Cytochrome bc1 complex | Electron transfer | Matrix processing peptidase | Mitochondria | Proton transfer | Q-cycle | Qcr | Stigmatellin | Ubiquinone | Yeast
