1f57
From Proteopedia
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| - | [[Image:1f57.jpg|left|200px]] | + | [[Image:1f57.jpg|left|200px]] |
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| - | '''CARBOXYPEPTIDASE A COMPLEX WITH D-CYSTEINE AT 1.75 A''' | + | {{Structure |
| + | |PDB= 1f57 |SIZE=350|CAPTION= <scene name='initialview01'>1f57</scene>, resolution 1.75Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene> and <scene name='pdbligand=DCY:D-CYSTEINE'>DCY</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Carboxypeptidase_A Carboxypeptidase A], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.17.1 3.4.17.1] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''CARBOXYPEPTIDASE A COMPLEX WITH D-CYSTEINE AT 1.75 A''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1F57 is a [ | + | 1F57 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F57 OCA]. |
==Reference== | ==Reference== | ||
| - | Crystal structure of carboxypeptidase A complexed with D-cysteine at 1.75 A - inhibitor-induced conformational changes., van Aalten DM, Chong CR, Joshua-Tor L, Biochemistry. 2000 Aug 22;39(33):10082-9. PMID:[http:// | + | Crystal structure of carboxypeptidase A complexed with D-cysteine at 1.75 A - inhibitor-induced conformational changes., van Aalten DM, Chong CR, Joshua-Tor L, Biochemistry. 2000 Aug 22;39(33):10082-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10955996 10955996] |
[[Category: Bos taurus]] | [[Category: Bos taurus]] | ||
[[Category: Carboxypeptidase A]] | [[Category: Carboxypeptidase A]] | ||
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[[Category: metalloprotease inhibitor]] | [[Category: metalloprotease inhibitor]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:04:35 2008'' |
Revision as of 09:04, 20 March 2008
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| , resolution 1.75Å | |||||||
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| Ligands: | and | ||||||
| Activity: | Carboxypeptidase A, with EC number 3.4.17.1 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
CARBOXYPEPTIDASE A COMPLEX WITH D-CYSTEINE AT 1.75 A
Overview
D-Cysteine differs from the antiarthritis drug D-penicillamine by only two methyl groups on the beta-carbon yet inhibits carboxypeptidase A (CPD) by a distinct mechanism: D-cysteine binds tightly to the active site zinc, while D-penicillamine catalyzes metal removal. To investigate the structural basis for this difference, we solved the crystal structure of carboxypeptidase A complexed with D-cysteine (D-Cys) at 1.75-A resolution. D-Cys binds the active site zinc with a sulfur ligand and forms additional interactions with surrounding side chains of the enzyme. The structure explains the difference in potency between D-Cys and L-Cys and provides insight into the mechanism of D-penicillamine inhibition. D-Cys binding induces a concerted motion of the side chains around the zinc ion, similar to that found in other carboxypeptidase-inhibitor crystal structures and along a limited path. Analysis of concerted motions of CPD and CPD-inhibitor crystal structures reveals a clustering of these structures into distinct groups. Using the restricted conformational flexibility of a drug target in this type of analysis could greatly enhance efficiency in drug design.
About this Structure
1F57 is a Single protein structure of sequence from Bos taurus. Full crystallographic information is available from OCA.
Reference
Crystal structure of carboxypeptidase A complexed with D-cysteine at 1.75 A - inhibitor-induced conformational changes., van Aalten DM, Chong CR, Joshua-Tor L, Biochemistry. 2000 Aug 22;39(33):10082-9. PMID:10955996
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