1o9i

From Proteopedia

Revision as of 13:42, 30 October 2007

CRYSTAL STRUCTURE OF THE Y42F MUTANT OF MANGANESE CATALASE FROM LACTOBACILLUS PLANTARUM AT 1.33A RESOLUTION

Overview

X-ray crystallography of the nonheme manganese catalase from Lactobacillus, plantarum (LPC) [Barynin, V.V., Whittaker, M.M., Antonyuk, S.V., Lamzin, V.S., Harrison, P.M., Artymiuk, P.J. & Whittaker, J.W. (2001) Structure9, 725-738] has revealed the structure of the dimanganese redox cluster, together with its protein environment. The oxidized [Mn(III)Mn(III)], cluster is bridged by two solvent molecules (oxo and hydroxo, respectively) together with a micro 1,3 bridging glutamate carboxylate and, is embedded in a web of hydrogen bonds involving an outer sphere tyrosine, residue (Tyr42). A novel homologous expression system has been developed, for production of active recombinant LPC and Tyr42 has been replaced by, phenylalanine using site-directed mutagenesis. Spectroscopic and, ... [(full description)]

About this Structure

1O9I is a [Single protein] structure of sequence from [Lactobacillus plantarum] with CA, NA, MN3, O and MES as [ligands]. Active as [Catalase], with EC number [1.11.1.6]. Structure known Active Site: BND. Full crystallographic information is available from [OCA].

Reference

Outer sphere mutagenesis of Lactobacillus plantarum manganese catalase disrupts the cluster core. Mechanistic implications., Whittaker MM, Barynin VV, Igarashi T, Whittaker JW, Eur J Biochem. 2003 Mar;270(6):1102-16. PMID:12631270

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