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1fhz
From Proteopedia
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| - | [[Image:1fhz.gif|left|200px]] | + | [[Image:1fhz.gif|left|200px]] |
| - | + | ||
| - | '''PSORALEN CROSS-LINKED D(CCGGTACCGG) FORMS HOLLIDAY JUNCTION''' | + | {{Structure |
| + | |PDB= 1fhz |SIZE=350|CAPTION= <scene name='initialview01'>1fhz</scene>, resolution 2.20Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=PSO:4'-HYDROXYMETHYL-4,5',8-TRIMETHYLPSORALEN'>PSO</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''PSORALEN CROSS-LINKED D(CCGGTACCGG) FORMS HOLLIDAY JUNCTION''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1FHZ is a [ | + | 1FHZ is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FHZ OCA]. |
==Reference== | ==Reference== | ||
| - | The crystal structures of psoralen cross-linked DNAs: drug-dependent formation of Holliday junctions., Eichman BF, Mooers BH, Alberti M, Hearst JE, Ho PS, J Mol Biol. 2001 Apr 20;308(1):15-26. PMID:[http:// | + | The crystal structures of psoralen cross-linked DNAs: drug-dependent formation of Holliday junctions., Eichman BF, Mooers BH, Alberti M, Hearst JE, Ho PS, J Mol Biol. 2001 Apr 20;308(1):15-26. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11302703 11302703] |
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Alberti, M.]] | [[Category: Alberti, M.]] | ||
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[[Category: psoralen]] | [[Category: psoralen]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:09:19 2008'' |
Revision as of 09:09, 20 March 2008
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| , resolution 2.20Å | |||||||
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| Ligands: | |||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
PSORALEN CROSS-LINKED D(CCGGTACCGG) FORMS HOLLIDAY JUNCTION
Overview
The single-crystal structures are presented for two DNA sequences with the thymine bases covalently cross-linked across the complementary strands by 4'-hydroxymethyl-4,5',8-trimethylpsoralen (HMT). The HMT-adduct of d(CCGCTAGCGG) forms a psoralen-induced Holliday junction, showing for the first time the effect of this important class of chemotheraputics on the structure of the recombination intermediate. In contrast, HMT-d(CCGGTACCGG) forms a sequence-dependent junction. In both structures, the DNA duplex is highly distorted at the thymine base linked to the six-member pyrone ring of the drug. The psoralen cross-link defines the intramolecular interactions of the drug-induced junction, while the sequence-dependent structure is nearly identical to the native Holliday junction of d(CCGGTACCGG) alone. The two structures contrast the effects of drug- and sequence-dependent interactions on the structure of a Holliday junction, suggesting a role for psoralen in the mechanism to initiate repair of psoralen-lesions in mammalian DNA.
About this Structure
1FHZ is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
The crystal structures of psoralen cross-linked DNAs: drug-dependent formation of Holliday junctions., Eichman BF, Mooers BH, Alberti M, Hearst JE, Ho PS, J Mol Biol. 2001 Apr 20;308(1):15-26. PMID:11302703
Page seeded by OCA on Thu Mar 20 11:09:19 2008
