1fp3
From Proteopedia
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| - | [[Image:1fp3.jpg|left|200px]] | + | [[Image:1fp3.jpg|left|200px]] |
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| - | '''CRYSTAL STRUCTURE OF N-ACYL-D-GLUCOSAMINE 2-EPIMERASE FROM PORCINE KIDNEY''' | + | {{Structure |
| + | |PDB= 1fp3 |SIZE=350|CAPTION= <scene name='initialview01'>1fp3</scene>, resolution 2.00Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/N-acylglucosamine_2-epimerase N-acylglucosamine 2-epimerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.1.3.8 5.1.3.8] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''CRYSTAL STRUCTURE OF N-ACYL-D-GLUCOSAMINE 2-EPIMERASE FROM PORCINE KIDNEY''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1FP3 is a [ | + | 1FP3 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FP3 OCA]. |
==Reference== | ==Reference== | ||
| - | Crystal structure of N-acyl-D-glucosamine 2-epimerase from porcine kidney at 2.0 A resolution., Itoh T, Mikami B, Maru I, Ohta Y, Hashimoto W, Murata K, J Mol Biol. 2000 Nov 10;303(5):733-44. PMID:[http:// | + | Crystal structure of N-acyl-D-glucosamine 2-epimerase from porcine kidney at 2.0 A resolution., Itoh T, Mikami B, Maru I, Ohta Y, Hashimoto W, Murata K, J Mol Biol. 2000 Nov 10;303(5):733-44. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11061972 11061972] |
[[Category: N-acylglucosamine 2-epimerase]] | [[Category: N-acylglucosamine 2-epimerase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: n-acyl-d-glucosamine 2-epimerase]] | [[Category: n-acyl-d-glucosamine 2-epimerase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:12:05 2008'' |
Revision as of 09:12, 20 March 2008
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| , resolution 2.00Å | |||||||
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| Activity: | N-acylglucosamine 2-epimerase, with EC number 5.1.3.8 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
CRYSTAL STRUCTURE OF N-ACYL-D-GLUCOSAMINE 2-EPIMERASE FROM PORCINE KIDNEY
Overview
The X-ray crystallographic structure of N-acyl-d-glucosamine 2-epimerase (AGE) from porcine kidney, which has been identified to be a renin-binding protein (RnBP), was determined by the multiple isomorphous replacement method and refined at 2.0 A resolution with a final R-factor of 16.9 % for 15 to 2.0 A resolution data. The refined structure of AGE comprised 804 amino acid residues (one dimer) and 145 water molecules. The dimer of AGE had an asymmetric unit with approximate dimensions 46 Ax48 Ax96 A. The AGE monomer is composed of an alpha(6)/alpha(6)-barrel, the structure of which is found in glucoamylase and cellulase. One side of the AGE alpha(6)/alpha(6)-barrel structure comprises long loops containing five short beta-sheets, and contributes to the formation of a deep cleft shaped like a funnel. The putative active-site pocket and a possible binding site for the substrate N-acetyl-d-glucosamine (GlcNAc) were found in the cleft. The other side of the alpha(6)/alpha(6)-barrel comprises short loops and contributes to the dimer formation. At the dimer interface, which is composed of the short loops and alpha-helices of the subunits, five strong ion-pair interactions were observed, which play a major role in the dimer assembly. This completely ruled out the previously accepted hypothesis that the formation of the RnBP homodimer and RnBP-renin heterodimer requires the leucine zipper motif present in RnBP.
About this Structure
1FP3 is a Single protein structure of sequence from Sus scrofa. Full crystallographic information is available from OCA.
Reference
Crystal structure of N-acyl-D-glucosamine 2-epimerase from porcine kidney at 2.0 A resolution., Itoh T, Mikami B, Maru I, Ohta Y, Hashimoto W, Murata K, J Mol Biol. 2000 Nov 10;303(5):733-44. PMID:11061972
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