1fwx
From Proteopedia
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| - | [[Image:1fwx.jpg|left|200px]] | + | [[Image:1fwx.jpg|left|200px]] |
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| - | '''CRYSTAL STRUCTURE OF NITROUS OXIDE REDUCTASE FROM P. DENITRIFICANS''' | + | {{Structure |
| + | |PDB= 1fwx |SIZE=350|CAPTION= <scene name='initialview01'>1fwx</scene>, resolution 1.60Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CUA:DINUCLEAR+COPPER+ION'>CUA</scene> and <scene name='pdbligand=CUZ:(MU-4-SULFIDO)-TETRA-NUCLEAR COPPER ION'>CUZ</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Nitrous-oxide_reductase Nitrous-oxide reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.7.99.6 1.7.99.6] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''CRYSTAL STRUCTURE OF NITROUS OXIDE REDUCTASE FROM P. DENITRIFICANS''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1FWX is a [ | + | 1FWX is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Paracoccus_denitrificans Paracoccus denitrificans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FWX OCA]. |
==Reference== | ==Reference== | ||
| - | Revisiting the catalytic CuZ cluster of nitrous oxide (N2O) reductase. Evidence of a bridging inorganic sulfur., Brown K, Djinovic-Carugo K, Haltia T, Cabrito I, Saraste M, Moura JJ, Moura I, Tegoni M, Cambillau C, J Biol Chem. 2000 Dec 29;275(52):41133-6. PMID:[http:// | + | Revisiting the catalytic CuZ cluster of nitrous oxide (N2O) reductase. Evidence of a bridging inorganic sulfur., Brown K, Djinovic-Carugo K, Haltia T, Cabrito I, Saraste M, Moura JJ, Moura I, Tegoni M, Cambillau C, J Biol Chem. 2000 Dec 29;275(52):41133-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11024061 11024061] |
[[Category: Nitrous-oxide reductase]] | [[Category: Nitrous-oxide reductase]] | ||
[[Category: Paracoccus denitrificans]] | [[Category: Paracoccus denitrificans]] | ||
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[[Category: cuz site]] | [[Category: cuz site]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:15:04 2008'' |
Revision as of 09:15, 20 March 2008
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| , resolution 1.60Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , and | ||||||
| Activity: | Nitrous-oxide reductase, with EC number 1.7.99.6 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
CRYSTAL STRUCTURE OF NITROUS OXIDE REDUCTASE FROM P. DENITRIFICANS
Overview
Nitrous-oxide reductases (N2OR) catalyze the two-electron reduction of N(2)O to N(2). The crystal structure of N2ORs from Pseudomonas nautica (Pn) and Paracoccus denitrificans (Pd) were solved at resolutions of 2.4 and 1.6 A, respectively. The Pn N2OR structure revealed that the catalytic CuZ center belongs to a new type of metal cluster in which four copper ions are liganded by seven histidine residues. A bridging oxygen moiety and two other hydroxide ligands were proposed to complete the ligation scheme (Brown, K., Tegoni, M., Prudencio, M., Pereira, A. S., Besson, S., Moura, J. J. G., Moura, I., and Cambillau, C. (2000) Nat. Struct. Biol. 7, 191-195). However, in the CuZ cluster, inorganic sulfur chemical determination and the high resolution structure of Pd N2OR identified a bridging inorganic sulfur instead of an oxygen. This result reconciles the novel CuZ cluster with the hitherto puzzling spectroscopic data.
About this Structure
1FWX is a Single protein structure of sequence from Paracoccus denitrificans. Full crystallographic information is available from OCA.
Reference
Revisiting the catalytic CuZ cluster of nitrous oxide (N2O) reductase. Evidence of a bridging inorganic sulfur., Brown K, Djinovic-Carugo K, Haltia T, Cabrito I, Saraste M, Moura JJ, Moura I, Tegoni M, Cambillau C, J Biol Chem. 2000 Dec 29;275(52):41133-6. PMID:11024061
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