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1g8z

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[[Image:1g8z.jpg|left|200px]]<br /><applet load="1g8z" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1g8z.jpg|left|200px]]
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caption="1g8z, resolution 2.0000&Aring;" />
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'''HIS57ALA MUTANT OF CHOLERA TOXIN B-PENATMER'''<br />
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{{Structure
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|PDB= 1g8z |SIZE=350|CAPTION= <scene name='initialview01'>1g8z</scene>, resolution 2.0000&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>
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|ACTIVITY=
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|GENE= CTXB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=666 Vibrio cholerae])
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}}
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'''HIS57ALA MUTANT OF CHOLERA TOXIN B-PENATMER'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1G8Z is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae] with <scene name='pdbligand=GAL:'>GAL</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G8Z OCA].
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1G8Z is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G8Z OCA].
==Reference==
==Reference==
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A mutant cholera toxin B subunit that binds GM1- ganglioside but lacks immunomodulatory or toxic activity., Aman AT, Fraser S, Merritt EA, Rodigherio C, Kenny M, Ahn M, Hol WG, Williams NA, Lencer WI, Hirst TR, Proc Natl Acad Sci U S A. 2001 Jul 17;98(15):8536-41. Epub 2001 Jul 10. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11447291 11447291]
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A mutant cholera toxin B subunit that binds GM1- ganglioside but lacks immunomodulatory or toxic activity., Aman AT, Fraser S, Merritt EA, Rodigherio C, Kenny M, Ahn M, Hol WG, Williams NA, Lencer WI, Hirst TR, Proc Natl Acad Sci U S A. 2001 Jul 17;98(15):8536-41. Epub 2001 Jul 10. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11447291 11447291]
[[Category: Single protein]]
[[Category: Single protein]]
[[Category: Vibrio cholerae]]
[[Category: Vibrio cholerae]]
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[[Category: toxin]]
[[Category: toxin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:47:27 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:19:53 2008''

Revision as of 09:19, 20 March 2008

Image:1g8z.jpg


PDB ID 1g8z

Drag the structure with the mouse to rotate
, resolution 2.0000Å
Ligands:
Gene: CTXB (Vibrio cholerae)
Coordinates: save as pdb, mmCIF, xml



HIS57ALA MUTANT OF CHOLERA TOXIN B-PENATMER


Overview

GM1-ganglioside receptor binding by the B subunit of cholera toxin (CtxB) is widely accepted to initiate toxin action by triggering uptake and delivery of the toxin A subunit into cells. More recently, GM1 binding by isolated CtxB, or the related B subunit of Escherichia coli heat-labile enterotoxin (EtxB), has been found to modulate leukocyte function, resulting in the down-regulation of proinflammatory immune responses that cause autoimmune disorders such as rheumatoid arthritis and diabetes. Here, we demonstrate that GM1 binding, contrary to expectation, is not sufficient to initiate toxin action. We report the engineering and crystallographic structure of a mutant cholera toxin, with a His to Ala substitution in the B subunit at position 57. Whereas the mutant retained pentameric stability and high affinity binding to GM1-ganglioside, it had lost its immunomodulatory activity and, when part of the holotoxin complex, exhibited ablated toxicity. The implications of these findings on the mode of action of cholera toxin are discussed.

About this Structure

1G8Z is a Single protein structure of sequence from Vibrio cholerae. Full crystallographic information is available from OCA.

Reference

A mutant cholera toxin B subunit that binds GM1- ganglioside but lacks immunomodulatory or toxic activity., Aman AT, Fraser S, Merritt EA, Rodigherio C, Kenny M, Ahn M, Hol WG, Williams NA, Lencer WI, Hirst TR, Proc Natl Acad Sci U S A. 2001 Jul 17;98(15):8536-41. Epub 2001 Jul 10. PMID:11447291

Page seeded by OCA on Thu Mar 20 11:19:53 2008

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