1ga1
From Proteopedia
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| - | [[Image:1ga1.gif|left|200px]] | + | [[Image:1ga1.gif|left|200px]] |
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| - | '''CRYSTAL STRUCTURE ANALYSIS OF PSCP (PSEUDOMONAS SERINE-CARBOXYL PROTEINASE) COMPLEXED WITH A FRAGMENT OF IODOTYROSTATIN (THIS ENZYME RENAMED "SEDOLISIN" IN 2003)''' | + | {{Structure |
| + | |PDB= 1ga1 |SIZE=350|CAPTION= <scene name='initialview01'>1ga1</scene>, resolution 1.40Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene> and <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Pseudomonalisin Pseudomonalisin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.100 3.4.21.100] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''CRYSTAL STRUCTURE ANALYSIS OF PSCP (PSEUDOMONAS SERINE-CARBOXYL PROTEINASE) COMPLEXED WITH A FRAGMENT OF IODOTYROSTATIN (THIS ENZYME RENAMED "SEDOLISIN" IN 2003)''' | ||
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==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1GA1 is a [ | + | 1GA1 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_sp. Pseudomonas sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GA1 OCA]. |
==Reference== | ==Reference== | ||
| - | Practical experience with the use of halides for phasing macromolecular structures: a powerful tool for structural genomics., Dauter Z, Li M, Wlodawer A, Acta Crystallogr D Biol Crystallogr. 2001 Feb;57(Pt 2):239-49. PMID:[http:// | + | Practical experience with the use of halides for phasing macromolecular structures: a powerful tool for structural genomics., Dauter Z, Li M, Wlodawer A, Acta Crystallogr D Biol Crystallogr. 2001 Feb;57(Pt 2):239-49. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11173470 11173470] |
[[Category: Pseudomonalisin]] | [[Category: Pseudomonalisin]] | ||
[[Category: Pseudomonas sp.]] | [[Category: Pseudomonas sp.]] | ||
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[[Category: serine-carboxyl proteinase]] | [[Category: serine-carboxyl proteinase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:20:18 2008'' |
Revision as of 09:20, 20 March 2008
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| , resolution 1.40Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , and | ||||||
| Activity: | Pseudomonalisin, with EC number 3.4.21.100 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
CRYSTAL STRUCTURE ANALYSIS OF PSCP (PSEUDOMONAS SERINE-CARBOXYL PROTEINASE) COMPLEXED WITH A FRAGMENT OF IODOTYROSTATIN (THIS ENZYME RENAMED "SEDOLISIN" IN 2003)
Overview
The crystal structure of pepstatin-insensitive carboxyl proteinase (PCP) from Pseudomonas sp. 101, an enzyme with no overall sequence similarity to any other proteinases of known structure, was solved using crystals soaked in sodium bromide solution and then cryocooled. A data set collected at the bromine peak absorption wavelength was sufficient for calculation of an excellent map and the entire process of phasing and tracing the maps required almost no direct human intervention. The process of structure solution using single-wavelength data was compared with three-wavelength multiwavelength anomalous diffraction (MAD); although the latter resulted in slightly better maps, the use of this much more labor-intensive approach did not significantly improve the ability to solve the structure. The successful phasing approaches are compared with several less successful attempts utilizing other crystal forms of the enzyme and the practical aspects of the use of bromine as a heavy-atom derivative are discussed. In conclusion, the use of halides with single-wavelength diffraction data fulfills the requirements of being a first-choice method of high-throughput structure solution for the emerging field of structural genomics.
About this Structure
1GA1 is a Single protein structure of sequence from Pseudomonas sp.. Full crystallographic information is available from OCA.
Reference
Practical experience with the use of halides for phasing macromolecular structures: a powerful tool for structural genomics., Dauter Z, Li M, Wlodawer A, Acta Crystallogr D Biol Crystallogr. 2001 Feb;57(Pt 2):239-49. PMID:11173470
Page seeded by OCA on Thu Mar 20 11:20:18 2008
Categories: Pseudomonalisin | Pseudomonas sp. | Single protein | Dauter, Z. | Li, M. | Wlodawer, A. | CA | CL | GOL | Serine-carboxyl proteinase
