1gk4
From Proteopedia
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| - | [[Image:1gk4.gif|left|200px]] | + | [[Image:1gk4.gif|left|200px]] |
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| - | '''HUMAN VIMENTIN COIL 2B FRAGMENT (CYS2)''' | + | {{Structure |
| + | |PDB= 1gk4 |SIZE=350|CAPTION= <scene name='initialview01'>1gk4</scene>, resolution 2.30Å | ||
| + | |SITE= <scene name='pdbsite=STA:Coiled-Coil+Stutter'>STA</scene> | ||
| + | |LIGAND= <scene name='pdbligand=ACT:ACETATE ION'>ACT</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''HUMAN VIMENTIN COIL 2B FRAGMENT (CYS2)''' | ||
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==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1GK4 is a [ | + | 1GK4 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GK4 OCA]. |
==Reference== | ==Reference== | ||
| - | Conserved segments 1A and 2B of the intermediate filament dimer: their atomic structures and role in filament assembly., Strelkov SV, Herrmann H, Geisler N, Wedig T, Zimbelmann R, Aebi U, Burkhard P, EMBO J. 2002 Mar 15;21(6):1255-66. PMID:[http:// | + | Conserved segments 1A and 2B of the intermediate filament dimer: their atomic structures and role in filament assembly., Strelkov SV, Herrmann H, Geisler N, Wedig T, Zimbelmann R, Aebi U, Burkhard P, EMBO J. 2002 Mar 15;21(6):1255-66. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11889032 11889032] |
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: stutter]] | [[Category: stutter]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:24:10 2008'' |
Revision as of 09:24, 20 March 2008
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| , resolution 2.30Å | |||||||
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| Coordinates: | save as pdb, mmCIF, xml | ||||||
HUMAN VIMENTIN COIL 2B FRAGMENT (CYS2)
Contents |
Overview
Intermediate filaments (IFs) are key components of the cytoskeleton in higher eukaryotic cells. The elementary IF 'building block' is an elongated coiled-coil dimer consisting of four consecutive alpha-helical segments. The segments 1A and 2B include highly conserved sequences and are critically involved in IF assembly. Based on the crystal structures of three human vimentin fragments at 1.4-2.3 A resolution (PDB entries 1gk4, 1gk6 and 1gk7), we have established the molecular organization of these two segments. The fragment corresponding to segment 1A forms a single, amphipatic alpha-helix, which is compatible with a coiled-coil geometry. While this segment might yield a coiled coil within an isolated dimer, monomeric 1A helices are likely to play a role in specific dimer-dimer interactions during IF assembly. The 2B segment reveals a double-stranded coiled coil, which unwinds near residue Phe351 to accommodate a 'stutter'. A fragment containing the last seven heptads of 2B interferes heavily with IF assembly and also transforms mature vimentin filaments into a new kind of structure. These results provide the first insight into the architecture and functioning of IFs at the atomic level.
Disease
Known disease associated with this structure: Inflammatory response, modulation of OMIM:[607918]
About this Structure
1GK4 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Conserved segments 1A and 2B of the intermediate filament dimer: their atomic structures and role in filament assembly., Strelkov SV, Herrmann H, Geisler N, Wedig T, Zimbelmann R, Aebi U, Burkhard P, EMBO J. 2002 Mar 15;21(6):1255-66. PMID:11889032
Page seeded by OCA on Thu Mar 20 11:24:10 2008
