1gyk

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[[Image:1gyk.gif|left|200px]]<br /><applet load="1gyk" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1gyk.gif|left|200px]]
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caption="1gyk, resolution 2.2&Aring;" />
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'''SERUM AMYLOID P COMPONENT CO-CRYSTALLISED WITH MOBDG AT NEUTRAL PH'''<br />
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{{Structure
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|PDB= 1gyk |SIZE=350|CAPTION= <scene name='initialview01'>1gyk</scene>, resolution 2.2&Aring;
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|SITE= <scene name='pdbsite=CA1:Ca+Binding+Site+For+Chain+E'>CA1</scene>
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|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene> and <scene name='pdbligand=CDG:METHYL 4,6-O-[(1R)-1-CARBOXYETHYLIDENE]-BETA-D-GALACTOPYRANOSIDE'>CDG</scene>
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|ACTIVITY=
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|GENE=
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}}
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'''SERUM AMYLOID P COMPONENT CO-CRYSTALLISED WITH MOBDG AT NEUTRAL PH'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1GYK is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=CA:'>CA</scene> and <scene name='pdbligand=CDG:'>CDG</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Site: <scene name='pdbsite=CA1:Ca+Binding+Site+For+Chain+E'>CA1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GYK OCA].
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1GYK is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GYK OCA].
==Reference==
==Reference==
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The structures of crystalline complexes of human serum amyloid P component with its carbohydrate ligand, the cyclic pyruvate acetal of galactose., Thompson D, Pepys MB, Tickle I, Wood S, J Mol Biol. 2002 Jul 26;320(5):1081-6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12126626 12126626]
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The structures of crystalline complexes of human serum amyloid P component with its carbohydrate ligand, the cyclic pyruvate acetal of galactose., Thompson D, Pepys MB, Tickle I, Wood S, J Mol Biol. 2002 Jul 26;320(5):1081-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12126626 12126626]
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: polymorphism]]
[[Category: polymorphism]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 12:55:23 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:29:42 2008''

Revision as of 09:29, 20 March 2008

Image:1gyk.gif


PDB ID 1gyk

Drag the structure with the mouse to rotate
, resolution 2.2Å
Sites:
Ligands: and
Coordinates: save as pdb, mmCIF, xml



SERUM AMYLOID P COMPONENT CO-CRYSTALLISED WITH MOBDG AT NEUTRAL PH


Contents

Overview

Two monoclinic (P2(1)) crystal forms of human serum amyloid P component (SAP) in complex with the 4,6-pyruvate acetal of beta-D-galactose (MObetaDG) were prepared. Structure analysis by molecular replacement and refinement at 2.2A resolution revealed that crystal form 1 (a=95.76A, b=70.53A, c=103.41A, beta=96.80 degrees) contained a pentamer in the asymmetric unit with a structure very similar to that of the published search model. The mode of ligand co-ordination was also similar except that four of the five subunits showed bound ligand with an additional H-bond between O1 of the galactose and the side-chain of Lys79. One sub-unit showed no bound ligand and a vacant calcium site close to a crystal contact. The 2.6A resolution structure of crystal form 2 (a=118.60A, b=109.10A, c=120.80A and beta=95.16 degrees ) showed ten sub-units in the asymmetric unit, all with two bound calcium ions and ligand. The most extensive protein-protein interactions between pentamers describe an AB face-to-face interaction involving 15 ion pairs that sandwiches five molecules of bound MObetaDG at the interface.

Disease

Known disease associated with this structure: Amyloidosis, secondary, susceptibility to OMIM:[104770]

About this Structure

1GYK is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

The structures of crystalline complexes of human serum amyloid P component with its carbohydrate ligand, the cyclic pyruvate acetal of galactose., Thompson D, Pepys MB, Tickle I, Wood S, J Mol Biol. 2002 Jul 26;320(5):1081-6. PMID:12126626

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