1gz5
From Proteopedia
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| - | [[Image:1gz5.gif|left|200px]] | + | [[Image:1gz5.gif|left|200px]] |
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| - | '''TREHALOSE-6-PHOSPHATE SYNTHASE. OTSA''' | + | {{Structure |
| + | |PDB= 1gz5 |SIZE=350|CAPTION= <scene name='initialview01'>1gz5</scene>, resolution 2.43Å | ||
| + | |SITE= <scene name='pdbsite=UDA:Imd+Binding+Site+For+Chain+D'>UDA</scene> | ||
| + | |LIGAND= <scene name='pdbligand=G6P:ALPHA-D-GLUCOSE-6-PHOSPHATE'>G6P</scene>, <scene name='pdbligand=UDP:URIDINE-5'-DIPHOSPHATE'>UDP</scene> and <scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Alpha,alpha-trehalose-phosphate_synthase_(UDP-forming) Alpha,alpha-trehalose-phosphate synthase (UDP-forming)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.15 2.4.1.15] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''TREHALOSE-6-PHOSPHATE SYNTHASE. OTSA''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1GZ5 is a [ | + | 1GZ5 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GZ5 OCA]. |
==Reference== | ==Reference== | ||
| - | Insights into trehalose synthesis provided by the structure of the retaining glucosyltransferase OtsA., Gibson RP, Turkenburg JP, Charnock SJ, Lloyd R, Davies GJ, Chem Biol. 2002 Dec;9(12):1337-46. PMID:[http:// | + | Insights into trehalose synthesis provided by the structure of the retaining glucosyltransferase OtsA., Gibson RP, Turkenburg JP, Charnock SJ, Lloyd R, Davies GJ, Chem Biol. 2002 Dec;9(12):1337-46. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12498887 12498887] |
[[Category: Alpha,alpha-trehalose-phosphate synthase (UDP-forming)]] | [[Category: Alpha,alpha-trehalose-phosphate synthase (UDP-forming)]] | ||
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
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[[Category: trehalose-6-phosphate]] | [[Category: trehalose-6-phosphate]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:29:58 2008'' |
Revision as of 09:29, 20 March 2008
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| , resolution 2.43Å | |||||||
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| Sites: | |||||||
| Ligands: | , and | ||||||
| Activity: | Alpha,alpha-trehalose-phosphate synthase (UDP-forming), with EC number 2.4.1.15 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
TREHALOSE-6-PHOSPHATE SYNTHASE. OTSA
Overview
Trehalose is a nonreducing disaccharide that plays a major role in many organisms, most notably in survival and stress responses. In Mycobacterium tuberculosis, it plays a central role as the carbohydrate core of numerous immunogenic glycolipids including "cord factor" (trehalose 6,6'-dimycolate). The classical pathway for trehalose synthesis involves the condensation of UDP-glucose and glucose-6-phosphate to afford trehalose-6-phosphate, catalyzed by the retaining glycosyltransferase OtsA. The configurations of two anomeric positions are set simultaneously, resulting in the formation of a double glycoside. The three-dimensional structure of the Escherichia coli OtsA, in complex with both UDP and glucose-6-phosphate, reveals the active site at the interface of two beta/alpha/beta domains. The overall structure and the intimate details of the catalytic machinery reveal a striking similarity to glycogen phosphorylase, indicating a strong evolutionary link and suggesting a common catalytic mechanism.
About this Structure
1GZ5 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Insights into trehalose synthesis provided by the structure of the retaining glucosyltransferase OtsA., Gibson RP, Turkenburg JP, Charnock SJ, Lloyd R, Davies GJ, Chem Biol. 2002 Dec;9(12):1337-46. PMID:12498887
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