1h6s
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:1h6s.gif|left|200px]] | + | [[Image:1h6s.gif|left|200px]] |
| - | + | ||
| - | '''ASYMMETRIC CONDUCTIVITY OF ENGINEERED PROTEINS''' | + | {{Structure |
| + | |PDB= 1h6s |SIZE=350|CAPTION= <scene name='initialview01'>1h6s</scene>, resolution 3.0Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''ASYMMETRIC CONDUCTIVITY OF ENGINEERED PROTEINS''' | ||
| + | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1H6S is a [ | + | 1H6S is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Rhodobacter_blasticus Rhodobacter blasticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H6S OCA]. |
==Reference== | ==Reference== | ||
| - | Asymmetric conductivity of engineered porins., Bannwarth M, Schulz GE, Protein Eng. 2002 Oct;15(10):799-804. PMID:[http:// | + | Asymmetric conductivity of engineered porins., Bannwarth M, Schulz GE, Protein Eng. 2002 Oct;15(10):799-804. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12468713 12468713] |
[[Category: Rhodobacter blasticus]] | [[Category: Rhodobacter blasticus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| Line 17: | Line 26: | ||
[[Category: integral membrane protein porin]] | [[Category: integral membrane protein porin]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:33:00 2008'' |
Revision as of 09:33, 20 March 2008
| |||||||
| , resolution 3.0Å | |||||||
|---|---|---|---|---|---|---|---|
| Coordinates: | save as pdb, mmCIF, xml | ||||||
ASYMMETRIC CONDUCTIVITY OF ENGINEERED PROTEINS
Overview
Positively charged peptide segments of 16 and 18 residues were inserted at a periplasmic turn of the porin from Rhodobacter blasticus in order to form an electric field-dependent plug. The X-ray diffraction analysis of a mutant confirmed that the structure of the porin had remained intact and that the insert was mobile. Incorporation experiments of single molecules into lipid bilayers showed that the distribution of electric conduction increments depended on the field polarity. The observed distributions are explained if the porin molecules enter the bilayer preferentially with their periplasmic surface first. Furthermore, the conduction of membrane-incorporated porin mutants changed reproducibly on field reversal showing asymmetries of reverse similar 15%, while the wild-type remained constant. This asymmetry is most likely caused by the electric field pressing the charged insert onto the pore eyelet in one field direction and removing it from the eyelet in the other. The results encourage attempts to improve the inserts in order to eventually reach diode characteristics.
About this Structure
1H6S is a Single protein structure of sequence from Rhodobacter blasticus. Full crystallographic information is available from OCA.
Reference
Asymmetric conductivity of engineered porins., Bannwarth M, Schulz GE, Protein Eng. 2002 Oct;15(10):799-804. PMID:12468713
Page seeded by OCA on Thu Mar 20 11:33:00 2008
