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1h7w
From Proteopedia
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| - | [[Image:1h7w.jpg|left|200px]] | + | [[Image:1h7w.jpg|left|200px]] |
| - | + | ||
| - | '''DIHYDROPYRIMIDINE DEHYDROGENASE (DPD) FROM PIG''' | + | {{Structure |
| + | |PDB= 1h7w |SIZE=350|CAPTION= <scene name='initialview01'>1h7w</scene>, resolution 1.90Å | ||
| + | |SITE= <scene name='pdbsite=AC5:Fmn+Binding+Site+For+Chain+A'>AC5</scene>, <scene name='pdbsite=AC6:Fad+Binding+Site+For+Chain+A'>AC6</scene>, <scene name='pdbsite=BC5:Fmn+Binding+Site+For+Chain+B'>BC5</scene>, <scene name='pdbsite=BC6:Fad+Binding+Site+For+Chain+B'>BC6</scene>, <scene name='pdbsite=CC5:Fmn+Binding+Site+For+Chain+C'>CC5</scene>, <scene name='pdbsite=CC6:Fad+Binding+Site+For+Chain+C'>CC6</scene>, <scene name='pdbsite=DC5:Fmn+Binding+Site+For+Chain+D'>DC5</scene> and <scene name='pdbsite=DC6:Fad+Binding+Site+For+Chain+D'>DC6</scene> | ||
| + | |LIGAND= <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene>, <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene> and <scene name='pdbligand=FAD:FLAVIN-ADENINE DINUCLEOTIDE'>FAD</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Dihydropyrimidine_dehydrogenase_(NADP(+)) Dihydropyrimidine dehydrogenase (NADP(+))], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.3.1.2 1.3.1.2] | ||
| + | |GENE= DPYD ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9823 Sus scrofa]) | ||
| + | }} | ||
| + | |||
| + | '''DIHYDROPYRIMIDINE DEHYDROGENASE (DPD) FROM PIG''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1H7W is a [ | + | 1H7W is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H7W OCA]. |
==Reference== | ==Reference== | ||
| - | Crystal structure of dihydropyrimidine dehydrogenase, a major determinant of the pharmacokinetics of the anti-cancer drug 5-fluorouracil., Dobritzsch D, Schneider G, Schnackerz KD, Lindqvist Y, EMBO J. 2001 Feb 15;20(4):650-60. PMID:[http:// | + | Crystal structure of dihydropyrimidine dehydrogenase, a major determinant of the pharmacokinetics of the anti-cancer drug 5-fluorouracil., Dobritzsch D, Schneider G, Schnackerz KD, Lindqvist Y, EMBO J. 2001 Feb 15;20(4):650-60. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11179210 11179210] |
[[Category: Dihydropyrimidine dehydrogenase (NADP(+))]] | [[Category: Dihydropyrimidine dehydrogenase (NADP(+))]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: electron transfer]] | [[Category: electron transfer]] | ||
[[Category: flavin]] | [[Category: flavin]] | ||
| - | [[Category: iron-sulfur | + | [[Category: iron-sulfur cluster]] |
[[Category: oxidoreductase]] | [[Category: oxidoreductase]] | ||
[[Category: pyrimidine catabolism]] | [[Category: pyrimidine catabolism]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:33:30 2008'' |
Revision as of 09:33, 20 March 2008
| |||||||
| , resolution 1.90Å | |||||||
|---|---|---|---|---|---|---|---|
| Sites: | , , , , , , and | ||||||
| Ligands: | , and | ||||||
| Gene: | DPYD (Sus scrofa) | ||||||
| Activity: | Dihydropyrimidine dehydrogenase (NADP(+)), with EC number 1.3.1.2 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
DIHYDROPYRIMIDINE DEHYDROGENASE (DPD) FROM PIG
Overview
Dihydropyrimidine dehydrogenase catalyzes the first step in pyrimidine degradation: the NADPH-dependent reduction of uracil and thymine to the corresponding 5,6-dihydropyrimidines. Its controlled inhibition has become an adjunct target for cancer therapy, since the enzyme is also responsible for the rapid breakdown of the chemotherapeutic drug 5-fluorouracil. The crystal structure of the homodimeric pig liver enzyme (2x 111 kDa) determined at 1.9 A resolution reveals a highly modular subunit organization, consisting of five domains with different folds. Dihydropyrimidine dehydrogenase contains two FAD, two FMN and eight [4Fe-4S] clusters, arranged in two electron transfer chains that pass the dimer interface twice. Two of the Fe-S clusters show a hitherto unobserved coordination involving a glutamine residue. The ternary complex of an inactive mutant of the enzyme with bound NADPH and 5-fluorouracil reveals the architecture of the substrate-binding sites and residues responsible for recognition and binding of the drug.
About this Structure
1H7W is a Single protein structure of sequence from Sus scrofa. Full crystallographic information is available from OCA.
Reference
Crystal structure of dihydropyrimidine dehydrogenase, a major determinant of the pharmacokinetics of the anti-cancer drug 5-fluorouracil., Dobritzsch D, Schneider G, Schnackerz KD, Lindqvist Y, EMBO J. 2001 Feb 15;20(4):650-60. PMID:11179210
Page seeded by OCA on Thu Mar 20 11:33:30 2008
