1h97
From Proteopedia
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| - | [[Image:1h97.gif|left|200px]] | + | [[Image:1h97.gif|left|200px]] |
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| - | '''TREMATODE HEMOGLOBIN FROM PARAMPHISTOMUM EPICLITUM''' | + | {{Structure |
| + | |PDB= 1h97 |SIZE=350|CAPTION= <scene name='initialview01'>1h97</scene>, resolution 1.17Å | ||
| + | |SITE= <scene name='pdbsite=HEA:Hem+Binding+Site+For+Chain+A'>HEA</scene> and <scene name='pdbsite=HEB:Hem+Binding+Site+For+Chain+B'>HEB</scene> | ||
| + | |LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> and <scene name='pdbligand=HEM:PROTOPORPHYRIN IX CONTAINING FE'>HEM</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''TREMATODE HEMOGLOBIN FROM PARAMPHISTOMUM EPICLITUM''' | ||
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==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1H97 is a [ | + | 1H97 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Paramphistomum_epiclitum Paramphistomum epiclitum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H97 OCA]. |
==Reference== | ==Reference== | ||
| - | Very high resolution structure of a trematode hemoglobin displaying a TyrB10-TyrE7 heme distal residue pair and high oxygen affinity., Pesce A, Dewilde S, Kiger L, Milani M, Ascenzi P, Marden MC, Van Hauwaert ML, Vanfleteren J, Moens L, Bolognesi M, J Mol Biol. 2001 Jun 22;309(5):1153-64. PMID:[http:// | + | Very high resolution structure of a trematode hemoglobin displaying a TyrB10-TyrE7 heme distal residue pair and high oxygen affinity., Pesce A, Dewilde S, Kiger L, Milani M, Ascenzi P, Marden MC, Van Hauwaert ML, Vanfleteren J, Moens L, Bolognesi M, J Mol Biol. 2001 Jun 22;309(5):1153-64. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11399085 11399085] |
[[Category: Paramphistomum epiclitum]] | [[Category: Paramphistomum epiclitum]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: non-vertebrate hemoglobin]] | [[Category: non-vertebrate hemoglobin]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:33:59 2008'' |
Revision as of 09:33, 20 March 2008
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| , resolution 1.17Å | |||||||
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| Sites: | and | ||||||
| Ligands: | and | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
TREMATODE HEMOGLOBIN FROM PARAMPHISTOMUM EPICLITUM
Overview
Monomeric hemoglobin from the trematode Paramphistomum epiclitum displays very high oxygen affinity (P(50)<0.001 mm Hg) and an unusual heme distal site containing tyrosyl residues at the B10 and E7 positions. The crystal structure of aquo-met P. epiclitum hemoglobin, solved at 1.17 A resolution via multiwavelength anomalous dispersion techniques (R-factor=0.121), shows that the heme distal site pocket residue TyrB10 is engaged in hydrogen bonding to the iron-bound ligand. By contrast, residue TyrE7 is unexpectedly locked next to the CD globin region, in a conformation unsuitable for heme-bound ligand stabilisation. Such structural organization of the E7 distal residue differs strikingly from that observed in the nematode Ascaris suum hemoglobin (bearing TyrB10 and GlnE7 residues), which also displays very high oxygen affinity. The oxygenation and carbonylation parameters of wild-type P. epiclitum Hb as well as of single- and double-site mutants, with residue substitutions at positions B10, E7 and E11, have been determined and are discussed here in the light of the protein atomic resolution crystal structure.
About this Structure
1H97 is a Single protein structure of sequence from Paramphistomum epiclitum. Full crystallographic information is available from OCA.
Reference
Very high resolution structure of a trematode hemoglobin displaying a TyrB10-TyrE7 heme distal residue pair and high oxygen affinity., Pesce A, Dewilde S, Kiger L, Milani M, Ascenzi P, Marden MC, Van Hauwaert ML, Vanfleteren J, Moens L, Bolognesi M, J Mol Biol. 2001 Jun 22;309(5):1153-64. PMID:11399085
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