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1hkc
From Proteopedia
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| - | [[Image:1hkc.gif|left|200px]] | + | [[Image:1hkc.gif|left|200px]] |
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| - | '''RECOMBINANT HUMAN HEXOKINASE TYPE I COMPLEXED WITH GLUCOSE AND PHOSPHATE''' | + | {{Structure |
| + | |PDB= 1hkc |SIZE=350|CAPTION= <scene name='initialview01'>1hkc</scene>, resolution 2.80Å | ||
| + | |SITE= <scene name='pdbsite=GLN:Glc+Binding+Site+In+N-Terminal+Domain'>GLN</scene>, <scene name='pdbsite=MEC:Metal+Ion+Binding+Site+In+C-Terminal+Domain'>MEC</scene>, <scene name='pdbsite=MEN:Metal+Ion+Binding+Site+In+N-Terminal+Domain'>MEN</scene>, <scene name='pdbsite=PIC:Phosphate+Binding+Site+In+C-Terminal+Domain'>PIC</scene> and <scene name='pdbsite=PIN:Phosphate+Binding+Site+In+N-Terminal+Domain'>PIN</scene> | ||
| + | |LIGAND= <scene name='pdbligand=GLC:GLUCOSE'>GLC</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene> and <scene name='pdbligand=K:POTASSIUM ION'>K</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Hexokinase Hexokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.1 2.7.1.1] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''RECOMBINANT HUMAN HEXOKINASE TYPE I COMPLEXED WITH GLUCOSE AND PHOSPHATE''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1HKC is a [ | + | 1HKC is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HKC OCA]. |
==Reference== | ==Reference== | ||
| - | Regulation of hexokinase I: crystal structure of recombinant human brain hexokinase complexed with glucose and phosphate., Aleshin AE, Zeng C, Bartunik HD, Fromm HJ, Honzatko RB, J Mol Biol. 1998 Sep 18;282(2):345-57. PMID:[http:// | + | Regulation of hexokinase I: crystal structure of recombinant human brain hexokinase complexed with glucose and phosphate., Aleshin AE, Zeng C, Bartunik HD, Fromm HJ, Honzatko RB, J Mol Biol. 1998 Sep 18;282(2):345-57. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9735292 9735292] |
[[Category: Hexokinase]] | [[Category: Hexokinase]] | ||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
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[[Category: phosphotransferase]] | [[Category: phosphotransferase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:38:13 2008'' |
Revision as of 09:38, 20 March 2008
| |||||||
| , resolution 2.80Å | |||||||
|---|---|---|---|---|---|---|---|
| Sites: | , , , and | ||||||
| Ligands: | , and | ||||||
| Activity: | Hexokinase, with EC number 2.7.1.1 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
RECOMBINANT HUMAN HEXOKINASE TYPE I COMPLEXED WITH GLUCOSE AND PHOSPHATE
Contents |
Overview
Hexokinase I, the pacemaker of glycolysis in brain tissue and red blood cells, is comprised of two similar domains fused into a single polypeptide chain. The C-terminal half of hexokinase I is catalytically active, whereas the N-terminal half is necessary for the relief of product inhibition by phosphate. A crystalline complex of recombinant human hexokinase I with glucose and phosphate (2.8 A resolution) reveals a single binding site for phosphate and glucose at the N-terminal half of the enzyme. Glucose and phosphate stabilize the N-terminal half in a closed conformation. Unexpectedly, glucose binds weakly to the C-terminal half of the enzyme and does not by itself stabilize a closed conformation. Evidently a stable, closed C-terminal half requires either ATP or glucose 6-phosphate along with glucose. The crystal structure here, in conjunction with other studies in crystallography and directed mutation, puts the phosphate regulatory site at the N-terminal half, the site of potent product inhibition at the C-terminal half, and a secondary site for the weak interaction of glucose 6-phosphate at the N-terminal half of the enzyme. The relevance of crystal structures of hexokinase I to the properties of monomeric hexokinase I and oligomers of hexokinase I bound to the surface of mitochondria is discussed.
Disease
Known disease associated with this structure: Hemolytic anemia due to hexokinase deficiency OMIM:[142600]
About this Structure
1HKC is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Regulation of hexokinase I: crystal structure of recombinant human brain hexokinase complexed with glucose and phosphate., Aleshin AE, Zeng C, Bartunik HD, Fromm HJ, Honzatko RB, J Mol Biol. 1998 Sep 18;282(2):345-57. PMID:9735292
Page seeded by OCA on Thu Mar 20 11:38:13 2008
Categories: Hexokinase | Homo sapiens | Single protein | Aleshin, A E. | Honzatko, R B. | GLC | K | PO4 | Allosteric enzyme | Glucose | Glycolysis | Phosphate | Phosphotransferase
