1hq3

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[[Image:1hq3.gif|left|200px]]<br /><applet load="1hq3" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1hq3.gif|left|200px]]
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caption="1hq3, resolution 2.15&Aring;" />
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'''CRYSTAL STRUCTURE OF THE HISTONE-CORE-OCTAMER IN KCL/PHOSPHATE'''<br />
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{{Structure
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|PDB= 1hq3 |SIZE=350|CAPTION= <scene name='initialview01'>1hq3</scene>, resolution 2.15&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene> and <scene name='pdbligand=CL:CHLORIDE ION'>CL</scene>
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|ACTIVITY=
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|GENE=
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}}
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'''CRYSTAL STRUCTURE OF THE HISTONE-CORE-OCTAMER IN KCL/PHOSPHATE'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1HQ3 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus] with <scene name='pdbligand=PO4:'>PO4</scene> and <scene name='pdbligand=CL:'>CL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HQ3 OCA].
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1HQ3 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HQ3 OCA].
==Reference==
==Reference==
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Structure of the histone-core octamer in KCl/phosphate crystals at 2.15 A resolution., Chantalat L, Nicholson JM, Lambert SJ, Reid AJ, Donovan MJ, Reynolds CD, Wood CM, Baldwin JP, Acta Crystallogr D Biol Crystallogr. 2003 Aug;59(Pt 8):1395-407. Epub 2003, Jul 23. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12876341 12876341]
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Structure of the histone-core octamer in KCl/phosphate crystals at 2.15 A resolution., Chantalat L, Nicholson JM, Lambert SJ, Reid AJ, Donovan MJ, Reynolds CD, Wood CM, Baldwin JP, Acta Crystallogr D Biol Crystallogr. 2003 Aug;59(Pt 8):1395-407. Epub 2003, Jul 23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12876341 12876341]
[[Category: Gallus gallus]]
[[Category: Gallus gallus]]
[[Category: Protein complex]]
[[Category: Protein complex]]
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[[Category: PO4]]
[[Category: PO4]]
[[Category: chromatin]]
[[Category: chromatin]]
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[[Category: cl and phosphate ions]]
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[[Category: cl and phosphate ion]]
[[Category: histone-core octamer]]
[[Category: histone-core octamer]]
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[[Category: histone-tail guide helices]]
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[[Category: histone-tail guide helice]]
[[Category: nucleosome]]
[[Category: nucleosome]]
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[[Category: structural elements]]
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[[Category: structural element]]
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[[Category: tether structures]]
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[[Category: tether structure]]
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[[Category: waters]]
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[[Category: water]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:03:41 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:40:11 2008''

Revision as of 09:40, 20 March 2008

Image:1hq3.gif


PDB ID 1hq3

Drag the structure with the mouse to rotate
, resolution 2.15Å
Ligands: and
Coordinates: save as pdb, mmCIF, xml



CRYSTAL STRUCTURE OF THE HISTONE-CORE-OCTAMER IN KCL/PHOSPHATE


Overview

The structure of the native chicken histone octamer, crystallized in 2 M KCl, 1.35 M potassium phosphate pH 6.9, has been refined at 2.15 A resolution to a final R factor of 21.4% and an R(free) of 25.2%. Unique crystal-packing interactions between histone-core octamers are strong and one of them (area 4000 A(2)) involves two chloride ions and direct interactions between six acidic amino-acid residues on one octamer and the equivalent number of basic residues on the next. These interactions are on the structured part of the octamer (not involving tails). Five phosphate ions, 23 chloride ions and 437 water molecules have been identified in the structure. The phosphate and some chloride ions bind to basic amino-acid residues that interact with DNA in the nucleosome. The binding of most of the anions and the packing interactions are unique to these crystals. In other respects, and including the positions of four chloride ions, the octamer structure is very close to that of octamers in nucleosome-core particle crystals, particularly with respect to 'docking' sequences of the histone H2As and H4s. These sequences together with the H2B-H4 four-helix bundles stabilize the histone structure in the nucleosome and prevent the dissociation of the (H2A-H2B) dimers from the (H3-H4)(2) tetramer. Possible reasons why this happens at high salt in the absence of DNA are given.

About this Structure

1HQ3 is a Protein complex structure of sequences from Gallus gallus. Full crystallographic information is available from OCA.

Reference

Structure of the histone-core octamer in KCl/phosphate crystals at 2.15 A resolution., Chantalat L, Nicholson JM, Lambert SJ, Reid AJ, Donovan MJ, Reynolds CD, Wood CM, Baldwin JP, Acta Crystallogr D Biol Crystallogr. 2003 Aug;59(Pt 8):1395-407. Epub 2003, Jul 23. PMID:12876341

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