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1hux

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[[Image:1hux.gif|left|200px]]<br /><applet load="1hux" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1hux.gif|left|200px]]
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caption="1hux, resolution 3.0&Aring;" />
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'''CRYSTAL STRUCTURE OF THE ACIDAMINOCOCCUS FERMENTANS (R)-2-HYDROXYGLUTARYL-COA DEHYDRATASE COMPONENT A'''<br />
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{{Structure
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|PDB= 1hux |SIZE=350|CAPTION= <scene name='initialview01'>1hux</scene>, resolution 3.0&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene> and <scene name='pdbligand=ADP:ADENOSINE-5'-DIPHOSPHATE'>ADP</scene>
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|ACTIVITY=
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|GENE= HGDC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=905 Acidaminococcus fermentans])
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}}
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'''CRYSTAL STRUCTURE OF THE ACIDAMINOCOCCUS FERMENTANS (R)-2-HYDROXYGLUTARYL-COA DEHYDRATASE COMPONENT A'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1HUX is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Acidaminococcus_fermentans Acidaminococcus fermentans] with <scene name='pdbligand=SF4:'>SF4</scene> and <scene name='pdbligand=ADP:'>ADP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HUX OCA].
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1HUX is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Acidaminococcus_fermentans Acidaminococcus fermentans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HUX OCA].
==Reference==
==Reference==
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Crystal structure of the Acidaminococcus fermentans 2-hydroxyglutaryl-CoA dehydratase component A., Locher KP, Hans M, Yeh AP, Schmid B, Buckel W, Rees DC, J Mol Biol. 2001 Mar 16;307(1):297-308. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11243821 11243821]
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Crystal structure of the Acidaminococcus fermentans 2-hydroxyglutaryl-CoA dehydratase component A., Locher KP, Hans M, Yeh AP, Schmid B, Buckel W, Rees DC, J Mol Biol. 2001 Mar 16;307(1):297-308. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11243821 11243821]
[[Category: Acidaminococcus fermentans]]
[[Category: Acidaminococcus fermentans]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: actin fold]]
[[Category: actin fold]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:05:04 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:41:54 2008''

Revision as of 09:41, 20 March 2008

Image:1hux.gif


PDB ID 1hux

Drag the structure with the mouse to rotate
, resolution 3.0Å
Ligands: and
Gene: HGDC (Acidaminococcus fermentans)
Coordinates: save as pdb, mmCIF, xml



CRYSTAL STRUCTURE OF THE ACIDAMINOCOCCUS FERMENTANS (R)-2-HYDROXYGLUTARYL-COA DEHYDRATASE COMPONENT A


Overview

Acidaminococcus fermentans degrades glutamate via the hydroxyglutarate pathway, which involves the syn-elimination of water from (R)-2-hydroxyglutaryl-CoA in a key reaction of the pathway. This anaerobic process is catalyzed by 2-hydroxyglutaryl-CoA dehydratase, an enzyme with two components (A and D) that reversibly associate during reaction cycles. Component A (CompA), a homodimeric protein of 2x27 kDa, contains a single, bridging [4Fe-4S] cluster and uses the hydrolysis of ATP to deliver an electron to the dehydratase component (CompD), where the electron is used catalytically. The structure of the extremely oxygen-sensitive CompA protein was solved by X-ray crystallography to 3 A resolution. The protein was found to be a member of the actin fold family, revealing a similar architecture and nucleotide-binding site. The key differences between CompA and other members of the actin fold family are: (i) the presence of a cluster binding segment, the "cluster helix"; (ii) the [4Fe-4S] cluster; and (iii) the location of the homodimer interface, which involves the bridging cluster. Possible reaction mechanisms are discussed in light of the close structural similarity to members of the actin-fold family and the functional similarity to the nitrogenase Fe- protein.

About this Structure

1HUX is a Single protein structure of sequence from Acidaminococcus fermentans. Full crystallographic information is available from OCA.

Reference

Crystal structure of the Acidaminococcus fermentans 2-hydroxyglutaryl-CoA dehydratase component A., Locher KP, Hans M, Yeh AP, Schmid B, Buckel W, Rees DC, J Mol Biol. 2001 Mar 16;307(1):297-308. PMID:11243821

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