This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1i25
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:1i25.jpg|left|200px]] | + | [[Image:1i25.jpg|left|200px]] |
| - | + | ||
| - | '''Three dimensional solution structure of huwentoxin-II by 2D 1H-NMR''' | + | {{Structure |
| + | |PDB= 1i25 |SIZE=350|CAPTION= <scene name='initialview01'>1i25</scene> | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''Three dimensional solution structure of huwentoxin-II by 2D 1H-NMR''' | ||
| + | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1I25 is a [ | + | 1I25 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Ornithoctonus_huwena Ornithoctonus huwena]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I25 OCA]. |
==Reference== | ==Reference== | ||
| - | The structure of spider toxin huwentoxin-II with unique disulfide linkage: evidence for structural evolution., Shu Q, Lu SY, Gu XC, Liang SP, Protein Sci. 2002 Feb;11(2):245-52. PMID:[http:// | + | The structure of spider toxin huwentoxin-II with unique disulfide linkage: evidence for structural evolution., Shu Q, Lu SY, Gu XC, Liang SP, Protein Sci. 2002 Feb;11(2):245-52. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11790834 11790834] |
[[Category: Ornithoctonus huwena]] | [[Category: Ornithoctonus huwena]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| Line 17: | Line 26: | ||
[[Category: Lu, S Y.]] | [[Category: Lu, S Y.]] | ||
[[Category: Shu, Q.]] | [[Category: Shu, Q.]] | ||
| - | [[Category: disulfide | + | [[Category: disulfide bond]] |
[[Category: insecticidal toxin]] | [[Category: insecticidal toxin]] | ||
[[Category: neurotoxin]] | [[Category: neurotoxin]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:44:31 2008'' |
Revision as of 09:44, 20 March 2008
| |||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Three dimensional solution structure of huwentoxin-II by 2D 1H-NMR
Overview
The three-dimensional structure of huwentoxin-II (HWTX-II), an insecticidal peptide purified from the venom of spider Selenocosmia huwena with a unique disulfide bond linkage as I-III, II-V, and IV-VI, has been determined using 2D (1)H-NMR. The resulting structure of HWTX-II contains two beta-turns (C4-S7 and K24-W27) and a double-stranded antiparallel beta-sheet (W27-C29 and C34-K36). Although the C-terminal double-stranded beta-sheet cross-linked by two disulfide bonds (II-V and IV-VI in HWTX-II, II-V and III-VI in the ICK molecules) is conserved both in HWTX-II and the ICK molecules, the structure of HWTX-II is unexpected absence of the cystine knot because of its unique disulfide linkage. It suggests that HWTX-II adopts a novel scaffold different from the ICK motif that is adopted by all other spider toxin structures elucidated thus far. Furthermore, the structure of HWTX-II, which conforms to the disulfide-directed beta-hairpin (DDH) motif, not only supports the hypothesis that the ICK is a minor elaboration of the more ancestral DDH motif but also suggests that HWTX-II may have evolved from the same structural ancestor.
About this Structure
1I25 is a Single protein structure of sequence from Ornithoctonus huwena. Full crystallographic information is available from OCA.
Reference
The structure of spider toxin huwentoxin-II with unique disulfide linkage: evidence for structural evolution., Shu Q, Lu SY, Gu XC, Liang SP, Protein Sci. 2002 Feb;11(2):245-52. PMID:11790834
Page seeded by OCA on Thu Mar 20 11:44:31 2008
