1i84
From Proteopedia
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| - | [[Image:1i84.gif|left|200px]] | + | [[Image:1i84.gif|left|200px]] |
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| - | '''CRYO-EM STRUCTURE OF THE HEAVY MEROMYOSIN SUBFRAGMENT OF CHICKEN GIZZARD SMOOTH MUSCLE MYOSIN WITH REGULATORY LIGHT CHAIN IN THE DEPHOSPHORYLATED STATE. ONLY C ALPHAS PROVIDED FOR REGULATORY LIGHT CHAIN. ONLY BACKBONE ATOMS PROVIDED FOR S2 FRAGMENT.''' | + | {{Structure |
| + | |PDB= 1i84 |SIZE=350|CAPTION= <scene name='initialview01'>1i84</scene>, resolution 20.0Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Myosin_ATPase Myosin ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.4.1 3.6.4.1] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''CRYO-EM STRUCTURE OF THE HEAVY MEROMYOSIN SUBFRAGMENT OF CHICKEN GIZZARD SMOOTH MUSCLE MYOSIN WITH REGULATORY LIGHT CHAIN IN THE DEPHOSPHORYLATED STATE. ONLY C ALPHAS PROVIDED FOR REGULATORY LIGHT CHAIN. ONLY BACKBONE ATOMS PROVIDED FOR S2 FRAGMENT.''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1I84 is a [ | + | 1I84 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I84 OCA]. |
==Reference== | ==Reference== | ||
| - | Three-dimensional image reconstruction of dephosphorylated smooth muscle heavy meromyosin reveals asymmetry in the interaction between myosin heads and placement of subfragment 2., Wendt T, Taylor D, Trybus KM, Taylor K, Proc Natl Acad Sci U S A. 2001 Apr 10;98(8):4361-6. Epub 2001 Apr 3. PMID:[http:// | + | Three-dimensional image reconstruction of dephosphorylated smooth muscle heavy meromyosin reveals asymmetry in the interaction between myosin heads and placement of subfragment 2., Wendt T, Taylor D, Trybus KM, Taylor K, Proc Natl Acad Sci U S A. 2001 Apr 10;98(8):4361-6. Epub 2001 Apr 3. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11287639 11287639] |
[[Category: Gallus gallus]] | [[Category: Gallus gallus]] | ||
[[Category: Myosin ATPase]] | [[Category: Myosin ATPase]] | ||
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[[Category: smooth muscle]] | [[Category: smooth muscle]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:46:49 2008'' |
Revision as of 09:46, 20 March 2008
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| , resolution 20.0Å | |||||||
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| Activity: | Myosin ATPase, with EC number 3.6.4.1 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
CRYO-EM STRUCTURE OF THE HEAVY MEROMYOSIN SUBFRAGMENT OF CHICKEN GIZZARD SMOOTH MUSCLE MYOSIN WITH REGULATORY LIGHT CHAIN IN THE DEPHOSPHORYLATED STATE. ONLY C ALPHAS PROVIDED FOR REGULATORY LIGHT CHAIN. ONLY BACKBONE ATOMS PROVIDED FOR S2 FRAGMENT.
Overview
Regulation of the actin-activated ATPase of smooth muscle myosin II is known to involve an interaction between the two heads that is controlled by phosphorylation of the regulatory light chain. However, the three-dimensional structure of this inactivated form has been unknown. We have used a lipid monolayer to obtain two-dimensional crystalline arrays of the unphosphorylated inactive form of smooth muscle heavy meromyosin suitable for structural studies by electron cryomicroscopy of unstained, frozen-hydrated specimens. The three-dimensional structure reveals an asymmetric interaction between the two myosin heads. The ATPase activity of one head is sterically "blocked" because part of its actin-binding interface is positioned onto the converter domain of the second head. ATPase activity of the second head, which can bind actin, appears to be inhibited through stabilization of converter domain movements needed to release phosphate and achieve strong actin binding. When the subfragment 2 domain of heavy meromyosin is oriented as it would be in an actomyosin filament lattice, the position of the heads is very different from that needed to bind actin, suggesting an additional contribution to ATPase inhibition in situ.
About this Structure
1I84 is a Protein complex structure of sequences from Gallus gallus. Full crystallographic information is available from OCA.
Reference
Three-dimensional image reconstruction of dephosphorylated smooth muscle heavy meromyosin reveals asymmetry in the interaction between myosin heads and placement of subfragment 2., Wendt T, Taylor D, Trybus KM, Taylor K, Proc Natl Acad Sci U S A. 2001 Apr 10;98(8):4361-6. Epub 2001 Apr 3. PMID:11287639
Page seeded by OCA on Thu Mar 20 11:46:49 2008
