1i9z

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[[Image:1i9z.jpg|left|200px]]<br /><applet load="1i9z" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1i9z.jpg|left|200px]]
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caption="1i9z, resolution 1.8&Aring;" />
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'''CRYSTAL STRUCTURE OF INOSITOL POLYPHOSPHATE 5-PHOSPHATASE DOMAIN (IPP5C) OF SPSYNAPTOJANIN IN COMPLEX WITH INOSITOL (1,4)-BISPHOSPHATE AND CALCIUM ION'''<br />
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{{Structure
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|PDB= 1i9z |SIZE=350|CAPTION= <scene name='initialview01'>1i9z</scene>, resolution 1.8&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene> and <scene name='pdbligand=2IP:D-MYO-INOSITOL-1,4-BISPHOSPHATE'>2IP</scene>
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|ACTIVITY=
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|GENE=
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}}
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'''CRYSTAL STRUCTURE OF INOSITOL POLYPHOSPHATE 5-PHOSPHATASE DOMAIN (IPP5C) OF SPSYNAPTOJANIN IN COMPLEX WITH INOSITOL (1,4)-BISPHOSPHATE AND CALCIUM ION'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1I9Z is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Schizosaccharomyces_pombe Schizosaccharomyces pombe] with <scene name='pdbligand=CA:'>CA</scene> and <scene name='pdbligand=2IP:'>2IP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I9Z OCA].
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1I9Z is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Schizosaccharomyces_pombe Schizosaccharomyces pombe]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I9Z OCA].
==Reference==
==Reference==
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Specificity determinants in phosphoinositide dephosphorylation: crystal structure of an archetypal inositol polyphosphate 5-phosphatase., Tsujishita Y, Guo S, Stolz LE, York JD, Hurley JH, Cell. 2001 May 4;105(3):379-89. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11348594 11348594]
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Specificity determinants in phosphoinositide dephosphorylation: crystal structure of an archetypal inositol polyphosphate 5-phosphatase., Tsujishita Y, Guo S, Stolz LE, York JD, Hurley JH, Cell. 2001 May 4;105(3):379-89. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11348594 11348594]
[[Category: Schizosaccharomyces pombe]]
[[Category: Schizosaccharomyces pombe]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: spsynaptojanin]]
[[Category: spsynaptojanin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:09:35 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:47:32 2008''

Revision as of 09:47, 20 March 2008

Image:1i9z.jpg


PDB ID 1i9z

Drag the structure with the mouse to rotate
, resolution 1.8Å
Ligands: and
Coordinates: save as pdb, mmCIF, xml



CRYSTAL STRUCTURE OF INOSITOL POLYPHOSPHATE 5-PHOSPHATASE DOMAIN (IPP5C) OF SPSYNAPTOJANIN IN COMPLEX WITH INOSITOL (1,4)-BISPHOSPHATE AND CALCIUM ION


Overview

Inositol polyphosphate 5-phosphatases are central to intracellular processes ranging from membrane trafficking to Ca(2+) signaling, and defects in this activity result in the human disease Lowe syndrome. The 1.8 resolution structure of the inositol polyphosphate 5-phosphatase domain of SPsynaptojanin bound to Ca(2+) and inositol (1,4)-bisphosphate reveals a fold and an active site His and Asp pair resembling those of several Mg(2+)-dependent nucleases. Additional loops mediate specific inositol polyphosphate contacts. The 4-phosphate of inositol (1,4)-bisphosphate is misoriented by 4.6 compared to the reactive geometry observed in the apurinic/apyrimidinic endonuclease 1, explaining the dephosphorylation site selectivity of the 5-phosphatases. Based on the structure, a series of mutants are described that exhibit altered substrate specificity providing general determinants for substrate recognition.

About this Structure

1I9Z is a Single protein structure of sequence from Schizosaccharomyces pombe. Full crystallographic information is available from OCA.

Reference

Specificity determinants in phosphoinositide dephosphorylation: crystal structure of an archetypal inositol polyphosphate 5-phosphatase., Tsujishita Y, Guo S, Stolz LE, York JD, Hurley JH, Cell. 2001 May 4;105(3):379-89. PMID:11348594

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