1w7z

From Proteopedia

Revision as of 14:23, 30 October 2007

CRYSTAL STRUCTURE OF THE FREE (UNCOMPLEXED) ECBALLIUM ELATERIUM TRYPSIN INHIBITOR (EETI-II)

Overview

The Ecballium elaterium trypsin inhibitor II (EETI-II) belongs to the, family of squash inhibitors and is one of the strongest inhibitors known, for trypsin. The eight independent molecules of EETI-II in the crystal, structure reported here provide a good opportunity to test the hypothesis, that this small cystine-knot protein (knottin) is sufficiently rigid to be, used as a molecular scaffold for protein-engineering purposes. To extend, this test, the structures of two complexes of EETI-II with trypsin have, also been determined, one carrying a four-amino-acid mutation of EETI-II., The remarkable similarity of these structures confirms the rigidity of the, molecular framework and hence its suitability as a molecular scaffold.

About this Structure

1W7Z is a [Single protein] structure of sequence from [Ecballium elaterium] with NA and FMT as [ligands]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].

Reference

Structure of Ecballium elaterium trypsin inhibitor II (EETI-II): a rigid molecular scaffold., Kratzner R, Debreczeni JE, Pape T, Schneider TR, Wentzel A, Kolmar H, Sheldrick GM, Uson I, Acta Crystallogr D Biol Crystallogr. 2005 Sep;61(Pt 9):1255-62. Epub 2005, Aug 16. PMID:16131759

Page seeded by OCA on Tue Oct 30 16:27:48 2007