1idq
From Proteopedia
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| - | [[Image:1idq.gif|left|200px]] | + | [[Image:1idq.gif|left|200px]] |
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| - | '''CRYSTAL STRUCTURE OF NATIVE VANADIUM-CONTAINING CHLOROPEROXIDASE FROM CURVULARIA INAEQUALIS''' | + | {{Structure |
| + | |PDB= 1idq |SIZE=350|CAPTION= <scene name='initialview01'>1idq</scene>, resolution 2.03Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=VO4:VANADATE ION'>VO4</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Chloride_peroxidase Chloride peroxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.10 1.11.1.10] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''CRYSTAL STRUCTURE OF NATIVE VANADIUM-CONTAINING CHLOROPEROXIDASE FROM CURVULARIA INAEQUALIS''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1IDQ is a [ | + | 1IDQ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Curvularia_inaequalis Curvularia inaequalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IDQ OCA]. |
==Reference== | ==Reference== | ||
| - | Implications for the catalytic mechanism of the vanadium-containing enzyme chloroperoxidase from the fungus Curvularia inaequalis by X-ray structures of the native and peroxide form., Messerschmidt A, Prade L, Wever R, Biol Chem. 1997 Mar-Apr;378(3-4):309-15. PMID:[http:// | + | Implications for the catalytic mechanism of the vanadium-containing enzyme chloroperoxidase from the fungus Curvularia inaequalis by X-ray structures of the native and peroxide form., Messerschmidt A, Prade L, Wever R, Biol Chem. 1997 Mar-Apr;378(3-4):309-15. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9165086 9165086] |
[[Category: Chloride peroxidase]] | [[Category: Chloride peroxidase]] | ||
[[Category: Curvularia inaequalis]] | [[Category: Curvularia inaequalis]] | ||
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[[Category: Wever, R.]] | [[Category: Wever, R.]] | ||
[[Category: VO4]] | [[Category: VO4]] | ||
| - | [[Category: two four-helix | + | [[Category: two four-helix bundle]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:48:59 2008'' |
Revision as of 09:48, 20 March 2008
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| , resolution 2.03Å | |||||||
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| Ligands: | |||||||
| Activity: | Chloride peroxidase, with EC number 1.11.1.10 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
CRYSTAL STRUCTURE OF NATIVE VANADIUM-CONTAINING CHLOROPEROXIDASE FROM CURVULARIA INAEQUALIS
Overview
Implications for the catalytic mechanism of the vanadium-containing chloroperoxidase from the fungus Curvularia inaequalis have been obtained from the crystal structures of the native and peroxide forms of the enzyme.The X-ray structures have been solved by difference Fourier techniques using the atomic model of the azide chloroperoxidase complex. The 2.03 A crystal structure (R = 19.7%) of the native enzyme reveals the geometry of the intact catalytic vanadium center. The vanadium is coordinated by four non-protein oxygen atoms and one nitrogen (NE2) atom from histidine 496 in a trigonal bipyramidal fashion. Three oxygens are in the equatorial plane and the fourth oxygen and the nitrogen are at the apexes of the bipyramid. In the 2.24 A crystal structure (R = 17.7%) of the peroxide derivate the peroxide is bound to the vanadium in an eta2-fashion after the release of the apical oxygen ligand. The vanadium is coordinated also by 4 non-protein oxygen atoms and one nitrogen (NE2) from histidine 496. The coordination geometry around the vanadium is that of a distorted tetragonal pyramid with the two peroxide oxygens, one oxygen and the nitrogen in the basal plane and one oxygen in the apical position. A mechanism for the catalytic cycle has been proposed based on these X-ray structures and kinetic data.
About this Structure
1IDQ is a Single protein structure of sequence from Curvularia inaequalis. Full crystallographic information is available from OCA.
Reference
Implications for the catalytic mechanism of the vanadium-containing enzyme chloroperoxidase from the fungus Curvularia inaequalis by X-ray structures of the native and peroxide form., Messerschmidt A, Prade L, Wever R, Biol Chem. 1997 Mar-Apr;378(3-4):309-15. PMID:9165086
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