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1idp
From Proteopedia
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| - | [[Image:1idp.gif|left|200px]] | + | [[Image:1idp.gif|left|200px]] |
| - | + | ||
| - | '''Crystal structure of scytalone dehydratase F162A mutant in the unligated state''' | + | {{Structure |
| + | |PDB= 1idp |SIZE=350|CAPTION= <scene name='initialview01'>1idp</scene>, resolution 1.45Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Scytalone_dehydratase Scytalone dehydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.94 4.2.1.94] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''Crystal structure of scytalone dehydratase F162A mutant in the unligated state''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1IDP is a [ | + | 1IDP is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Magnaporthe_grisea Magnaporthe grisea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IDP OCA]. |
==Reference== | ==Reference== | ||
| - | Crystallization of scytalone dehydratase F162A mutant in the unligated state and a preliminary X-ray diffraction study at 37 K., Motoyama T, Nakasako M, Yamaguchi I, Acta Crystallogr D Biol Crystallogr. 2002 Jan;58(Pt 1):148-50. Epub 2001, Dec 21. PMID:[http:// | + | Crystallization of scytalone dehydratase F162A mutant in the unligated state and a preliminary X-ray diffraction study at 37 K., Motoyama T, Nakasako M, Yamaguchi I, Acta Crystallogr D Biol Crystallogr. 2002 Jan;58(Pt 1):148-50. Epub 2001, Dec 21. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11752795 11752795] |
[[Category: Magnaporthe grisea]] | [[Category: Magnaporthe grisea]] | ||
[[Category: Scytalone dehydratase]] | [[Category: Scytalone dehydratase]] | ||
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[[Category: melanine biosynthesis]] | [[Category: melanine biosynthesis]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:48:59 2008'' |
Revision as of 09:49, 20 March 2008
| |||||||
| , resolution 1.45Å | |||||||
|---|---|---|---|---|---|---|---|
| Activity: | Scytalone dehydratase, with EC number 4.2.1.94 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of scytalone dehydratase F162A mutant in the unligated state
Overview
Scytalone dehydratase variant F162A, in which Phe162 in the C-terminal region was replaced with alanine, was crystallized with polyethylene glycol 4000. Because the crystal was radiation-sensitive, the diffraction data were collected at cryogenic temperatures. The crystal belonged to monoclinic space group P2(1), with unit-cell parameters a = 72.64, b = 61.30, c = 72.62 A, beta = 120.02 degrees at 37 K. The calculated V(M) value was acceptable when a trimer of the mutant enzyme occupied a crystallographic asymmetric unit. The resolution limit was extended to 1.45 A at BL41XU of SPring-8 at 37 K.
About this Structure
1IDP is a Single protein structure of sequence from Magnaporthe grisea. Full crystallographic information is available from OCA.
Reference
Crystallization of scytalone dehydratase F162A mutant in the unligated state and a preliminary X-ray diffraction study at 37 K., Motoyama T, Nakasako M, Yamaguchi I, Acta Crystallogr D Biol Crystallogr. 2002 Jan;58(Pt 1):148-50. Epub 2001, Dec 21. PMID:11752795
Page seeded by OCA on Thu Mar 20 11:48:59 2008
