1ihf

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1ihf.gif|left|200px]]<br /><applet load="1ihf" size="350" color="white" frame="true" align="right" spinBox="true"
+
[[Image:1ihf.gif|left|200px]]
-
caption="1ihf, resolution 2.200&Aring;" />
+
 
-
'''INTEGRATION HOST FACTOR/DNA COMPLEX'''<br />
+
{{Structure
 +
|PDB= 1ihf |SIZE=350|CAPTION= <scene name='initialview01'>1ihf</scene>, resolution 2.200&Aring;
 +
|SITE=
 +
|LIGAND= <scene name='pdbligand=CD:CADMIUM ION'>CD</scene>
 +
|ACTIVITY=
 +
|GENE=
 +
}}
 +
 
 +
'''INTEGRATION HOST FACTOR/DNA COMPLEX'''
 +
 
==Overview==
==Overview==
Line 7: Line 16:
==About this Structure==
==About this Structure==
-
1IHF is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=CD:'>CD</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IHF OCA].
+
1IHF is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IHF OCA].
==Reference==
==Reference==
-
Crystal structure of an IHF-DNA complex: a protein-induced DNA U-turn., Rice PA, Yang S, Mizuuchi K, Nash HA, Cell. 1996 Dec 27;87(7):1295-306. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8980235 8980235]
+
Crystal structure of an IHF-DNA complex: a protein-induced DNA U-turn., Rice PA, Yang S, Mizuuchi K, Nash HA, Cell. 1996 Dec 27;87(7):1295-306. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8980235 8980235]
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Protein complex]]
[[Category: Protein complex]]
Line 23: Line 32:
[[Category: transcription factor]]
[[Category: transcription factor]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:11:51 2008''
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:50:24 2008''

Revision as of 09:50, 20 March 2008

Image:1ihf.gif


PDB ID 1ihf

Drag the structure with the mouse to rotate
, resolution 2.200Å
Ligands:
Coordinates: save as pdb, mmCIF, xml



INTEGRATION HOST FACTOR/DNA COMPLEX


Overview

Integration host factor (IHF) is a small heterodimeric protein that specifically binds to DNA and functions as an architectural factor in many cellular processes in prokaryotes. Here, we report the crystal structure of IHF complexed with 35 bp of DNA. The DNA is wrapped around the protein and bent by >160 degrees, thus reversing the direction of the helix axis within a very short distance. Much of the bending occurs at two large kinks where the base stacking is interrupted by intercalation of a proline residue. IHF contacts the DNA exclusively via the phosphodiester backbone and the minor groove and relies heavily on indirect readout to recognize its binding sequence. One such readout involves a six-base A tract, providing evidence for the importance of a narrow minor groove.

About this Structure

1IHF is a Protein complex structure of sequences from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Crystal structure of an IHF-DNA complex: a protein-induced DNA U-turn., Rice PA, Yang S, Mizuuchi K, Nash HA, Cell. 1996 Dec 27;87(7):1295-306. PMID:8980235

Page seeded by OCA on Thu Mar 20 11:50:24 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1ihf"
Personal tools