This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1ybk
From Proteopedia
(Difference between revisions)
m (Protected "1ybk" [edit=sysop:move=sysop]) |
|||
| Line 1: | Line 1: | ||
| - | [[ | + | ==RHCC cocrystallized with CAPB== |
| + | <StructureSection load='1ybk' size='340' side='right' caption='[[1ybk]], [[Resolution|resolution]] 1.45Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[1ybk]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylothermus_marinus Staphylothermus marinus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YBK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1YBK FirstGlance]. <br> | ||
| + | </td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BEQ:N-(CARBOXYMETHYL)-N,N-DIMETHYL-3-[(1-OXODODECYL)AMINO]-1-PROPANAMINIUM+INNER+SALT'>BEQ</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br> | ||
| + | <tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1fe6|1fe6]]</td></tr> | ||
| + | <tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ybk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ybk OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ybk RCSB], [http://www.ebi.ac.uk/pdbsum/1ybk PDBsum]</span></td></tr> | ||
| + | <table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Crystals of excellent quality are a prerequisite for high-resolution X-ray data. However, in refinement protocols of crystallization conditions it is often difficult to obtain the right combination of, for example, protein concentration, drop size, temperature and additives. A novel approach for optimizing crystal contacts in a most favourable fashion by performing crystallization setups with the zwitterionic surfactant cocoamidoproylbetaine (CAPB) is introduced. In the presence of this surfactant, highly diffracting crystals were obtained. Here, data from a right-handed coiled coil (RHCC) in complex with CAPB at 1.4 A resolution are presented. The addition of CAPB using otherwise identical crystallization conditions and the same X-ray source caused an improvement in resolution from 2.9 to 1.4 A. | ||
| - | + | Favourable mediation of crystal contacts by cocoamidopropylbetaine (CAPB).,Ozbek S, Muller JF, Figgemeier E, Stetefeld J Acta Crystallogr D Biol Crystallogr. 2005 Apr;61(Pt 4):477-80. Epub 2005, Mar 24. PMID:15805603<ref>PMID:15805603</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | == References == | |
| - | + | <references/> | |
| - | + | __TOC__ | |
| - | + | </StructureSection> | |
| - | + | ||
| - | == | + | |
| - | < | + | |
[[Category: Staphylothermus marinus]] | [[Category: Staphylothermus marinus]] | ||
[[Category: Stetefeld, J.]] | [[Category: Stetefeld, J.]] | ||
[[Category: Protein binding]] | [[Category: Protein binding]] | ||
[[Category: Right hand coiled coil]] | [[Category: Right hand coiled coil]] | ||
Revision as of 07:08, 9 June 2014
RHCC cocrystallized with CAPB
| |||||||||||
