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1ilw

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[[Image:1ilw.gif|left|200px]]<br /><applet load="1ilw" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1ilw.gif|left|200px]]
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caption="1ilw, resolution 2.05&Aring;" />
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'''Crystal Structure of Pyrazinamidase/Nicotinamidase of Pyrococcus horikoshii'''<br />
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{{Structure
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|PDB= 1ilw |SIZE=350|CAPTION= <scene name='initialview01'>1ilw</scene>, resolution 2.05&Aring;
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|SITE=
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|LIGAND=
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|ACTIVITY= [http://en.wikipedia.org/wiki/Nicotinamidase Nicotinamidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.19 3.5.1.19]
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|GENE=
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}}
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'''Crystal Structure of Pyrazinamidase/Nicotinamidase of Pyrococcus horikoshii'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1ILW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pyrococcus_horikoshii Pyrococcus horikoshii]. Active as [http://en.wikipedia.org/wiki/Nicotinamidase Nicotinamidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.19 3.5.1.19] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ILW OCA].
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1ILW is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pyrococcus_horikoshii Pyrococcus horikoshii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ILW OCA].
==Reference==
==Reference==
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Crystal structure and mechanism of catalysis of a pyrazinamidase from Pyrococcus horikoshii., Du X, Wang W, Kim R, Yakota H, Nguyen H, Kim SH, Biochemistry. 2001 Nov 27;40(47):14166-72. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11714269 11714269]
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Crystal structure and mechanism of catalysis of a pyrazinamidase from Pyrococcus horikoshii., Du X, Wang W, Kim R, Yakota H, Nguyen H, Kim SH, Biochemistry. 2001 Nov 27;40(47):14166-72. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11714269 11714269]
[[Category: Nicotinamidase]]
[[Category: Nicotinamidase]]
[[Category: Pyrococcus horikoshii]]
[[Category: Pyrococcus horikoshii]]
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[[Category: pyrazinamidase]]
[[Category: pyrazinamidase]]
[[Category: pyrazinamide]]
[[Category: pyrazinamide]]
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[[Category: structural genomics]]
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[[Category: structural genomic]]
[[Category: tuberculosis]]
[[Category: tuberculosis]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:13:12 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:51:59 2008''

Revision as of 09:52, 20 March 2008

Image:1ilw.gif


PDB ID 1ilw

Drag the structure with the mouse to rotate
, resolution 2.05Å
Activity: Nicotinamidase, with EC number 3.5.1.19
Coordinates: save as pdb, mmCIF, xml



Crystal Structure of Pyrazinamidase/Nicotinamidase of Pyrococcus horikoshii


Overview

Bacterial pyrazinamidase (PZAase)/nicotinamidase converts pyrazinamide (PZA) to ammonia and pyrazinoic acid, which is active against Mycobacterium tuberculosis. Loss of PZAase activity is the major mechanism of pyrazinamide-resistance by M. tuberculosis. We have determined the crystal structure of the gene product of Pyrococcus horikoshii 999 (PH999), a PZAase, and its complex with zinc ion by X-ray crystallography. The overall fold of PH999 is similar to that of N-carbamoylsarcosine amidohydrolase (CSHase) of Arthrobacter sp. and YcaC of Escherichia coli, a protein with unknown physiological function. The active site of PH999 was identified by structural features that are also present in the active sites of CSHase and YcaC: a triad (D10, K94, and C133) and a cis-peptide (between V128 and A129). Surprisingly, a metal ion-binding site was revealed in the active site and subsequently confirmed by crystal structure of PH999 in complex with Zn(2+). The roles of the triad, cis-peptide, and metal ion in the catalysis are proposed. Because of extensive homology between PH999 and PZAase of M. tuberculosis (37% sequence identity), the structure of PH999 provides a structural basis for understanding PZA-resistance by M. tuberculosis harboring PZAase mutations.

About this Structure

1ILW is a Single protein structure of sequence from Pyrococcus horikoshii. Full crystallographic information is available from OCA.

Reference

Crystal structure and mechanism of catalysis of a pyrazinamidase from Pyrococcus horikoshii., Du X, Wang W, Kim R, Yakota H, Nguyen H, Kim SH, Biochemistry. 2001 Nov 27;40(47):14166-72. PMID:11714269

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