2pwt

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[[Image:2pwt.png|left|200px]]
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==Crystal structure of the bacterial ribosomal decoding site complexed with aminoglycoside containing the L-HABA group==
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<StructureSection load='2pwt' size='340' side='right' caption='[[2pwt]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2pwt]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PWT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2PWT FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=LHA:DOUBLY+FUNCTIONALIZED+PAROMOMYCIN+PM-II-162'>LHA</scene><br>
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2pwt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pwt OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2pwt RCSB], [http://www.ebi.ac.uk/pdbsum/2pwt PDBsum]</span></td></tr>
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<table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The crystal structure of the complex between oligonucleotide containing the bacterial ribosomal decoding site (A site) and the synthetic paromomycin analogue 1, which contains the gamma-amino-alpha-hydroxybutyryl (L-haba) group at position N1 of ring II (2-DOS ring), and an ether chain with an O-phenethylaminoethyl group at position C2'' of ring III, is reported. Interestingly, next to the paromomycin analogue 1 specifically bound to the A site, a second molecule of 1 with a different conformation is observed at the crystal packing interface which mimics the A-minor interaction between two bulged-out adenines from the A site and the codon-anticodon stem of the mRNA-tRNA complex. Improved antibacterial activity supports the conclusion that analogue 1 might affect protein synthesis on the ribosome in two different ways: 1) specific binding to the A site forces maintenance of the "on" state with two bulged out adenines, and 2) a new binding mode of 1 to an A-minor motif which stabilizes complex formation between the ribosome and the mRNA-tRNA complex regardless of whether the codon-anticodon stem is of the cognate or near-cognate type.
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{{STRUCTURE_2pwt| PDB=2pwt | SCENE= }}
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Crystal Structure of the Bacterial Ribosomal Decoding Site Complexed with a Synthetic Doubly Functionalized Paromomycin Derivative: a New Specific Binding Mode to an A-Minor Motif Enhances in vitro Antibacterial Activity.,Kondo J, Pachamuthu K, Francois B, Szychowski J, Hanessian S, Westhof E ChemMedChem. 2007 Nov 12;2(11):1631-1638. PMID:17722211<ref>PMID:17722211</ref>
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===Crystal structure of the bacterial ribosomal decoding site complexed with aminoglycoside containing the L-HABA group===
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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{{ABSTRACT_PUBMED_17722211}}
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== References ==
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<references/>
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==About this Structure==
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__TOC__
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[[2pwt]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PWT OCA].
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</StructureSection>
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==Reference==
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<ref group="xtra">PMID:017722211</ref><references group="xtra"/>
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[[Category: Francois, B.]]
[[Category: Francois, B.]]
[[Category: Hanessian, S.]]
[[Category: Hanessian, S.]]

Revision as of 07:12, 9 June 2014

Crystal structure of the bacterial ribosomal decoding site complexed with aminoglycoside containing the L-HABA group

2pwt, resolution 1.80Å

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