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1imx

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[[Image:1imx.gif|left|200px]]<br /><applet load="1imx" size="350" color="white" frame="true" align="right" spinBox="true"
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[[Image:1imx.gif|left|200px]]
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caption="1imx, resolution 1.82&Aring;" />
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'''1.8 Angstrom crystal structure of IGF-1'''<br />
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{{Structure
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|PDB= 1imx |SIZE=350|CAPTION= <scene name='initialview01'>1imx</scene>, resolution 1.82&Aring;
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|SITE=
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|LIGAND= <scene name='pdbligand=BR:BROMIDE+ION'>BR</scene> and <scene name='pdbligand=CPQ:N,N-BIS(3-D-GLUCONAMIDOPROPYL)DEOXYCHOLAMIDE'>CPQ</scene>
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|ACTIVITY=
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|GENE= IGF-1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
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}}
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'''1.8 Angstrom crystal structure of IGF-1'''
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==Overview==
==Overview==
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==About this Structure==
==About this Structure==
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1IMX is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=BR:'>BR</scene> and <scene name='pdbligand=CPQ:'>CPQ</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IMX OCA].
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1IMX is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IMX OCA].
==Reference==
==Reference==
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Crystal structure of human insulin-like growth factor-1: detergent binding inhibits binding protein interactions., Vajdos FF, Ultsch M, Schaffer ML, Deshayes KD, Liu J, Skelton NJ, de Vos AM, Biochemistry. 2001 Sep 18;40(37):11022-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11551198 11551198]
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Crystal structure of human insulin-like growth factor-1: detergent binding inhibits binding protein interactions., Vajdos FF, Ultsch M, Schaffer ML, Deshayes KD, Liu J, Skelton NJ, de Vos AM, Biochemistry. 2001 Sep 18;40(37):11022-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11551198 11551198]
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: insulin/relaxin]]
[[Category: insulin/relaxin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:13:34 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:52:21 2008''

Revision as of 09:52, 20 March 2008

Image:1imx.gif


PDB ID 1imx

Drag the structure with the mouse to rotate
, resolution 1.82Å
Ligands: and
Gene: IGF-1 (Homo sapiens)
Coordinates: save as pdb, mmCIF, xml



1.8 Angstrom crystal structure of IGF-1


Contents

Overview

Despite efforts spanning considerably more than a decade, a high-resolution view of the family of proteins known as insulin-like growth factors (IGFs) has remained elusive. IGF-1 consists of three helical segments which are connected by a 12-residue linker known as the C-region. NMR studies of members of this family reveal a dynamic structure with a topology resembling insulin but little structural definition in the C-region. We have crystallized IGF-1 in the presence of the detergent deoxy big CHAPS, and determined its structure at 1.8 A resolution by multiwavelength anomalous diffraction, exploiting the anomalous scattering of a single bromide ion and six of the seven sulfur atoms of IGF-1. The structure reveals a well-defined conformation for much of the C-region, which extends away from the core of IGF-1 and has residues known to be involved in receptor binding prominently displayed in a type II beta-turn. In the crystal, these residues form a dimer interface, but analytical ultracentrifugation experiments demonstrate that at physiological concentrations IGF-1 is monomeric. A single detergent molecule contacts residues known to be important for IGF-1 binding protein (IGFBP) interactions. Biophysical and biochemical data show that the detergent binds to IGF-1 specifically and blocks binding of IGFBP-1 and IGFBP-3.

Disease

Known disease associated with this structure: Growth retardation with deafness and mental retardation due to IGF1 deficiency OMIM:[147440]

About this Structure

1IMX is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Crystal structure of human insulin-like growth factor-1: detergent binding inhibits binding protein interactions., Vajdos FF, Ultsch M, Schaffer ML, Deshayes KD, Liu J, Skelton NJ, de Vos AM, Biochemistry. 2001 Sep 18;40(37):11022-9. PMID:11551198

Page seeded by OCA on Thu Mar 20 11:52:21 2008

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