1ip3
From Proteopedia
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| - | [[Image:1ip3.jpg|left|200px]] | + | [[Image:1ip3.jpg|left|200px]] |
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| - | '''G68A HUMAN LYSOZYME''' | + | {{Structure |
| + | |PDB= 1ip3 |SIZE=350|CAPTION= <scene name='initialview01'>1ip3</scene>, resolution 1.8Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=NA:SODIUM+ION'>NA</scene> and <scene name='pdbligand=SO4:SULFATE ION'>SO4</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''G68A HUMAN LYSOZYME''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1IP3 is a [ | + | 1IP3 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IP3 OCA]. |
==Reference== | ==Reference== | ||
| - | Role of amino acid residues in left-handed helical conformation for the conformational stability of a protein., Takano K, Yamagata Y, Yutani K, Proteins. 2001 Nov 15;45(3):274-80. PMID:[http:// | + | Role of amino acid residues in left-handed helical conformation for the conformational stability of a protein., Takano K, Yamagata Y, Yutani K, Proteins. 2001 Nov 15;45(3):274-80. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11599030 11599030] |
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Lysozyme]] | [[Category: Lysozyme]] | ||
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[[Category: glycosidase]] | [[Category: glycosidase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:53:11 2008'' |
Revision as of 09:53, 20 March 2008
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| , resolution 1.8Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | and | ||||||
| Activity: | Lysozyme, with EC number 3.2.1.17 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
G68A HUMAN LYSOZYME
Contents |
Overview
Our previous study of six non-Gly to Gly/Ala mutant human lysozymes in a left-handed helical region showed that only one non-Gly residue at a rigid site had unfavorable strain energy as compared with Gly at the same position (Takano et al., Proteins 2001; 44:233-243). To further examine the role of left-handed residues in the conformational stability of a protein, we constructed ten Gly to Ala mutant human lysozymes. Most Gly residues in human lysozyme are located in the left-handed helix region. The thermodynamic parameters for denaturation and crystal structures were determined by differential scanning calorimetry and X-ray analysis, respectively. The difference in denaturation Gibbs energy (DeltaDeltaG) for the ten Gly to Ala mutants ranged from + 1.9 to -7.5 kJ/mol, indicating that the effect of the mutation depends on the environment of the residue. We confirm that Gly in a left-handed region is more favorable at rigid sites than non-Gly, but there is little difference in energetic cost between Gly and non-Gly at flexible sites. The present results indicate that dihedral angles in the backbone conformation and also the flexibility at the position should be considered for analyses of protein stability, and protein structural determination, prediction, and design.
Disease
Known diseases associated with this structure: Amyloidosis, renal OMIM:[153450], Microphthalmia, syndromic 1 OMIM:[309800]
About this Structure
1IP3 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Role of amino acid residues in left-handed helical conformation for the conformational stability of a protein., Takano K, Yamagata Y, Yutani K, Proteins. 2001 Nov 15;45(3):274-80. PMID:11599030
Page seeded by OCA on Thu Mar 20 11:53:11 2008
Categories: Homo sapiens | Lysozyme | Single protein | Takano, K. | Yamagata, Y. | Yutani, K. | NA | SO4 | Bacteriolytic enzyme | Glycosidase
