This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1iw7
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:1iw7.gif|left|200px]] | + | [[Image:1iw7.gif|left|200px]] |
| - | + | ||
| - | '''Crystal structure of the RNA polymerase holoenzyme from Thermus thermophilus at 2.6A resolution''' | + | {{Structure |
| + | |PDB= 1iw7 |SIZE=350|CAPTION= <scene name='initialview01'>1iw7</scene>, resolution 2.6Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=PB:LEAD+(II)+ION'>PB</scene> and <scene name='pdbligand=MG:MAGNESIUM ION'>MG</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''Crystal structure of the RNA polymerase holoenzyme from Thermus thermophilus at 2.6A resolution''' | ||
| + | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1IW7 is a [ | + | 1IW7 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. The following page contains interesting information on the relation of 1IW7 with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb48_1.html Catabolite Activator Protein]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IW7 OCA]. |
==Reference== | ==Reference== | ||
| - | Crystal structure of a bacterial RNA polymerase holoenzyme at 2.6 A resolution., Vassylyev DG, Sekine S, Laptenko O, Lee J, Vassylyeva MN, Borukhov S, Yokoyama S, Nature. 2002 Jun 13;417(6890):712-9. Epub 2002 May 8. PMID:[http:// | + | Crystal structure of a bacterial RNA polymerase holoenzyme at 2.6 A resolution., Vassylyev DG, Sekine S, Laptenko O, Lee J, Vassylyeva MN, Borukhov S, Yokoyama S, Nature. 2002 Jun 13;417(6890):712-9. Epub 2002 May 8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12000971 12000971] |
[[Category: Catabolite Activator Protein]] | [[Category: Catabolite Activator Protein]] | ||
[[Category: DNA-directed RNA polymerase]] | [[Category: DNA-directed RNA polymerase]] | ||
| Line 21: | Line 30: | ||
[[Category: rna polymerase holoenzyme]] | [[Category: rna polymerase holoenzyme]] | ||
[[Category: rsgi]] | [[Category: rsgi]] | ||
| - | [[Category: structural | + | [[Category: structural genomic]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:55:41 2008'' |
Revision as of 09:55, 20 March 2008
| |||||||
| , resolution 2.6Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | and | ||||||
| Activity: | DNA-directed RNA polymerase, with EC number 2.7.7.6 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of the RNA polymerase holoenzyme from Thermus thermophilus at 2.6A resolution
Overview
In bacteria, the binding of a single protein, the initiation factor sigma, to a multi-subunit RNA polymerase core enzyme results in the formation of a holoenzyme, the active form of RNA polymerase essential for transcription initiation. Here we report the crystal structure of a bacterial RNA polymerase holoenzyme from Thermus thermophilus at 2.6 A resolution. In the structure, two amino-terminal domains of the sigma subunit form a V-shaped structure near the opening of the upstream DNA-binding channel of the active site cleft. The carboxy-terminal domain of sigma is near the outlet of the RNA-exit channel, about 57 A from the N-terminal domains. The extended linker domain forms a hairpin protruding into the active site cleft, then stretching through the RNA-exit channel to connect the N- and C-terminal domains. The holoenzyme structure provides insight into the structural organization of transcription intermediate complexes and into the mechanism of transcription initiation.
About this Structure
1IW7 is a Protein complex structure of sequences from Thermus thermophilus. The following page contains interesting information on the relation of 1IW7 with [Catabolite Activator Protein]. Full crystallographic information is available from OCA.
Reference
Crystal structure of a bacterial RNA polymerase holoenzyme at 2.6 A resolution., Vassylyev DG, Sekine S, Laptenko O, Lee J, Vassylyeva MN, Borukhov S, Yokoyama S, Nature. 2002 Jun 13;417(6890):712-9. Epub 2002 May 8. PMID:12000971
Page seeded by OCA on Thu Mar 20 11:55:41 2008
