1jgx
From Proteopedia
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| - | [[Image:1jgx.jpg|left|200px]] | + | [[Image:1jgx.jpg|left|200px]] |
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| - | '''Photosynthetic Reaction Center Mutant With Thr M 21 Replaced With Asp''' | + | {{Structure |
| + | |PDB= 1jgx |SIZE=350|CAPTION= <scene name='initialview01'>1jgx</scene>, resolution 3.01Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=BCL:BACTERIOCHLOROPHYLL+A'>BCL</scene>, <scene name='pdbligand=BPH:BACTERIOPHEOPHYTIN+A'>BPH</scene>, <scene name='pdbligand=U10:UBIQUINONE-10'>U10</scene> and <scene name='pdbligand=SPO:SPHEROIDENE'>SPO</scene> | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
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| + | '''Photosynthetic Reaction Center Mutant With Thr M 21 Replaced With Asp''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1JGX is a [ | + | 1JGX is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Rhodobacter_sphaeroides Rhodobacter sphaeroides]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JGX OCA]. |
==Reference== | ==Reference== | ||
| - | Individual interactions influence the crystalline order for membrane proteins., Camara-Artigas A, Magee CL, Williams JC, Allen JP, Acta Crystallogr D Biol Crystallogr. 2001 Sep;57(Pt 9):1281-6. Epub 2001, Aug 23. PMID:[http:// | + | Individual interactions influence the crystalline order for membrane proteins., Camara-Artigas A, Magee CL, Williams JC, Allen JP, Acta Crystallogr D Biol Crystallogr. 2001 Sep;57(Pt 9):1281-6. Epub 2001, Aug 23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11526320 11526320] |
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Rhodobacter sphaeroides]] | [[Category: Rhodobacter sphaeroides]] | ||
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[[Category: alpha helix]] | [[Category: alpha helix]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:03:14 2008'' |
Revision as of 10:03, 20 March 2008
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| , resolution 3.01Å | |||||||
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| Ligands: | , , , and | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Photosynthetic Reaction Center Mutant With Thr M 21 Replaced With Asp
Overview
The role of contact interactions in the crystallization of membrane proteins was assessed by mutation of amino-acid residues on the surface of the reaction center from Rhodobacter sphaeroides. Five single-site mutants were constructed, with changes in contact regions found in the trigonal and tetragonal forms but not the orthorhombic form. Crystallization trials for the tetragonal form yielded either no crystals or crystals with an altered morphology, whereas crystals grew in the other two forms, indicating that these interactions are essential for the stability of the tetragonal crystals. Changes in the structures determined by X-ray diffraction of trigonal crystals for each mutant were related to the quality of the diffraction. Significant differences in the resolution limit of the crystals were associated with the loss of specific interactions between neighboring proteins. The results suggest that the contact regions are crucial for obtaining highly ordered crystals of membrane proteins.
About this Structure
1JGX is a Protein complex structure of sequences from Rhodobacter sphaeroides. Full crystallographic information is available from OCA.
Reference
Individual interactions influence the crystalline order for membrane proteins., Camara-Artigas A, Magee CL, Williams JC, Allen JP, Acta Crystallogr D Biol Crystallogr. 2001 Sep;57(Pt 9):1281-6. Epub 2001, Aug 23. PMID:11526320
Page seeded by OCA on Thu Mar 20 12:03:14 2008
Categories: Protein complex | Rhodobacter sphaeroides | Allen, J P. | Camara-Artigas, A. | Magee, C L. | Williams, J C. | BCL | BPH | FE | SPO | U10 | Alpha helix
