1ji6
From Proteopedia
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| - | [[Image:1ji6.jpg|left|200px]] | + | [[Image:1ji6.jpg|left|200px]] |
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| - | '''CRYSTAL STRUCTURE OF THE INSECTICIDAL BACTERIAL DEL ENDOTOXIN CRY3Bb1 BACILLUS THURINGIENSIS''' | + | {{Structure |
| + | |PDB= 1ji6 |SIZE=350|CAPTION= <scene name='initialview01'>1ji6</scene>, resolution 2.4Å | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= | ||
| + | }} | ||
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| + | '''CRYSTAL STRUCTURE OF THE INSECTICIDAL BACTERIAL DEL ENDOTOXIN CRY3Bb1 BACILLUS THURINGIENSIS''' | ||
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==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1JI6 is a [ | + | 1JI6 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_thuringiensis Bacillus thuringiensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JI6 OCA]. |
==Reference== | ==Reference== | ||
| - | Structure of the insecticidal bacterial delta-endotoxin Cry3Bb1 of Bacillus thuringiensis., Galitsky N, Cody V, Wojtczak A, Ghosh D, Luft JR, Pangborn W, English L, Acta Crystallogr D Biol Crystallogr. 2001 Aug;57(Pt 8):1101-9. Epub 2001, Jul 23. PMID:[http:// | + | Structure of the insecticidal bacterial delta-endotoxin Cry3Bb1 of Bacillus thuringiensis., Galitsky N, Cody V, Wojtczak A, Ghosh D, Luft JR, Pangborn W, English L, Acta Crystallogr D Biol Crystallogr. 2001 Aug;57(Pt 8):1101-9. Epub 2001, Jul 23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11468393 11468393] |
[[Category: Bacillus thuringiensis]] | [[Category: Bacillus thuringiensis]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: cry3bb1]] | [[Category: cry3bb1]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:03:46 2008'' |
Revision as of 10:03, 20 March 2008
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CRYSTAL STRUCTURE OF THE INSECTICIDAL BACTERIAL DEL ENDOTOXIN CRY3Bb1 BACILLUS THURINGIENSIS
Overview
The coleopteran-active delta-endotoxin Cry3Bb1 from Bacillus thuringiensis (Bt) strain EG7231 is uniquely toxic to Diabrotica undecimpunctata, the Southern corn rootworm, while retaining activity against Leptinotarsa decemlineata, the Colorado potato beetle. The crystal structure of the delta-endotoxin Cry3Bb1 has been refined using data collected to 2.4 A resolution, with a residual R factor of 17.5% and an R(free) of 25.3%. The structure is made up of three domains: I, a seven-helix bundle (residues 64-294); II, a three-sheet domain (residues 295-502); and III, a beta-sandwich domain (residues 503-652). The monomers in the orthorhombic C222(1) crystal lattice form a dimeric quaternary structure across a crystallographic twofold axis, with a channel formed involving interactions between domains I and III. There are 23 hydrogen bonds between the two monomers conferring structural stability on the dimer. It has been demonstrated that Cry3Bb1 and the similar toxin Cry3A form oligomers in solution. The structural results presented here indicate that the interactions between domains I and III could be responsible for the initial higher order structure and have implications for the biological activity of these toxins. There are seven additional single amino-acid residues in the sequence of Cry3Bb1 compared with that of Cry3A; one in domain I, two in domain II and four in domain III, which also shows the largest conformational difference between the two proteins. These changes can be implicated in the selectivity differences noted for these two delta-endotoxins.
About this Structure
1JI6 is a Single protein structure of sequence from Bacillus thuringiensis. Full crystallographic information is available from OCA.
Reference
Structure of the insecticidal bacterial delta-endotoxin Cry3Bb1 of Bacillus thuringiensis., Galitsky N, Cody V, Wojtczak A, Ghosh D, Luft JR, Pangborn W, English L, Acta Crystallogr D Biol Crystallogr. 2001 Aug;57(Pt 8):1101-9. Epub 2001, Jul 23. PMID:11468393
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