1jof
From Proteopedia
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| - | [[Image:1jof.jpg|left|200px]] | + | [[Image:1jof.jpg|left|200px]] |
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| - | '''Neurospora crassa 3-carboxy-cis,cis-mucoante lactonizing enzyme''' | + | {{Structure |
| + | |PDB= 1jof |SIZE=350|CAPTION= <scene name='initialview01'>1jof</scene>, resolution 2.5Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=PIN:PIPERAZINE-N,N'-BIS(2-ETHANESULFONIC+ACID)'>PIN</scene> and <scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Carboxy-cis,cis-muconate_cyclase Carboxy-cis,cis-muconate cyclase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.5.1.5 5.5.1.5] | ||
| + | |GENE= | ||
| + | }} | ||
| + | |||
| + | '''Neurospora crassa 3-carboxy-cis,cis-mucoante lactonizing enzyme''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1JOF is a [ | + | 1JOF is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Neurospora_crassa Neurospora crassa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JOF OCA]. |
==Reference== | ==Reference== | ||
| - | The structure of Neurospora crassa 3-carboxy-cis,cis-muconate lactonizing enzyme, a beta propeller cycloisomerase., Kajander T, Merckel MC, Thompson A, Deacon AM, Mazur P, Kozarich JW, Goldman A, Structure. 2002 Apr;10(4):483-92. PMID:[http:// | + | The structure of Neurospora crassa 3-carboxy-cis,cis-muconate lactonizing enzyme, a beta propeller cycloisomerase., Kajander T, Merckel MC, Thompson A, Deacon AM, Mazur P, Kozarich JW, Goldman A, Structure. 2002 Apr;10(4):483-92. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11937053 11937053] |
[[Category: Carboxy-cis,cis-muconate cyclase]] | [[Category: Carboxy-cis,cis-muconate cyclase]] | ||
[[Category: Neurospora crassa]] | [[Category: Neurospora crassa]] | ||
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[[Category: semet-protein]] | [[Category: semet-protein]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:06:11 2008'' |
Revision as of 10:06, 20 March 2008
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| , resolution 2.5Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , and | ||||||
| Activity: | Carboxy-cis,cis-muconate cyclase, with EC number 5.5.1.5 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Neurospora crassa 3-carboxy-cis,cis-mucoante lactonizing enzyme
Overview
Muconate lactonizing enzymes (MLEs) convert cis,cis-muconates to muconolactones in microbes as part of the beta-ketoadipate pathway; some also dehalogenate muconate derivatives of xenobiotic haloaromatics. There are three different MLE classes unrelated by evolution. We present the X-ray structure of a eukaryotic MLE, Neurospora crassa 3-carboxy-cis,cis-muconate lactonizing enzyme (NcCMLE) at 2.5 A resolution, with a seven-bladed beta propeller fold. It is related neither to bacterial MLEs nor to other beta propeller enzymes, but is structurally similar to the G protein beta subunit. It reveals a novel metal-independent cycloisomerase motif unlike the bacterial metal cofactor MLEs. Together, the bacterial MLEs and NcCMLE structures comprise a striking structural example of functional convergence in enzymes for 1,2-addition-elimination of carboxylic acids. NcCMLE and bacterial MLEs may enhance the reaction rate differently: the former by electrophilic catalysis and the latter by electrostatic stabilization of the enolate.
About this Structure
1JOF is a Single protein structure of sequence from Neurospora crassa. Full crystallographic information is available from OCA.
Reference
The structure of Neurospora crassa 3-carboxy-cis,cis-muconate lactonizing enzyme, a beta propeller cycloisomerase., Kajander T, Merckel MC, Thompson A, Deacon AM, Mazur P, Kozarich JW, Goldman A, Structure. 2002 Apr;10(4):483-92. PMID:11937053
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