1jqr
From Proteopedia
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| - | [[Image:1jqr.jpg|left|200px]] | + | [[Image:1jqr.jpg|left|200px]] |
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| - | '''NMR structure of the African swine fever virus DNA polymerase X''' | + | {{Structure |
| + | |PDB= 1jqr |SIZE=350|CAPTION= <scene name='initialview01'>1jqr</scene> | ||
| + | |SITE= | ||
| + | |LIGAND= | ||
| + | |ACTIVITY= | ||
| + | |GENE= O174L ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10497 African swine fever virus]) | ||
| + | }} | ||
| + | |||
| + | '''NMR structure of the African swine fever virus DNA polymerase X''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1JQR is a [ | + | 1JQR is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/African_swine_fever_virus African swine fever virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JQR OCA]. |
==Reference== | ==Reference== | ||
| - | Solution structure of a viral DNA polymerase X and evidence for a mutagenic function., Showalter AK, Byeon IJ, Su MI, Tsai MD, Nat Struct Biol. 2001 Nov;8(11):942-6. PMID:[http:// | + | Solution structure of a viral DNA polymerase X and evidence for a mutagenic function., Showalter AK, Byeon IJ, Su MI, Tsai MD, Nat Struct Biol. 2001 Nov;8(11):942-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11685239 11685239] |
[[Category: African swine fever virus]] | [[Category: African swine fever virus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: dna polymerase]] | [[Category: dna polymerase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:07:08 2008'' |
Revision as of 10:07, 20 March 2008
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| Gene: | O174L (African swine fever virus) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
NMR structure of the African swine fever virus DNA polymerase X
Overview
The African swine fever virus DNA polymerase X (ASFV Pol X or Pol X), the smallest known nucleotide polymerase, has recently been reported to be an extremely low fidelity polymerase that may be involved in strategic mutagenesis of the viral genome. Here we report the solution structure of Pol X. The structure, unique within the realm of nucleotide polymerases, consists of only palm and fingers subdomains. Despite the absence of a thumb subdomain, which is important for DNA binding in other polymerases, we show that Pol X binds DNA with very high affinity. Further structural analyses suggest a novel mode of DNA binding that may contribute to low fidelity synthesis. We also demonstrate that the ASFV DNA ligase is a low fidelity ligase capable of sealing a nick that contains a G-G mismatch. This supports the hypothesis of a virus-encoded, mutagenic base excision repair pathway consisting of a tandem Pol X/ligase mutator.
About this Structure
1JQR is a Single protein structure of sequence from African swine fever virus. Full crystallographic information is available from OCA.
Reference
Solution structure of a viral DNA polymerase X and evidence for a mutagenic function., Showalter AK, Byeon IJ, Su MI, Tsai MD, Nat Struct Biol. 2001 Nov;8(11):942-6. PMID:11685239
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