1jsc
From Proteopedia
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| - | [[Image:1jsc.gif|left|200px]] | + | [[Image:1jsc.gif|left|200px]] |
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| - | '''Crystal Structure of the Catalytic Subunit of Yeast Acetohydroxyacid Synthase: A target for Herbicidal Inhibitors''' | + | {{Structure |
| + | |PDB= 1jsc |SIZE=350|CAPTION= <scene name='initialview01'>1jsc</scene>, resolution 2.6Å | ||
| + | |SITE= | ||
| + | |LIGAND= <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=2HP:DIHYDROGENPHOSPHATE+ION'>2HP</scene>, <scene name='pdbligand=TPP:THIAMINE+DIPHOSPHATE'>TPP</scene> and <scene name='pdbligand=FAD:FLAVIN-ADENINE DINUCLEOTIDE'>FAD</scene> | ||
| + | |ACTIVITY= [http://en.wikipedia.org/wiki/Acetolactate_synthase Acetolactate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.2.1.6 2.2.1.6] | ||
| + | |GENE= ILV2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]) | ||
| + | }} | ||
| + | |||
| + | '''Crystal Structure of the Catalytic Subunit of Yeast Acetohydroxyacid Synthase: A target for Herbicidal Inhibitors''' | ||
| + | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1JSC is a [ | + | 1JSC is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JSC OCA]. |
==Reference== | ==Reference== | ||
| - | Crystal structure of yeast acetohydroxyacid synthase: a target for herbicidal inhibitors., Pang SS, Duggleby RG, Guddat LW, J Mol Biol. 2002 Mar 22;317(2):249-62. PMID:[http:// | + | Crystal structure of yeast acetohydroxyacid synthase: a target for herbicidal inhibitors., Pang SS, Duggleby RG, Guddat LW, J Mol Biol. 2002 Mar 22;317(2):249-62. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11902841 11902841] |
[[Category: Acetolactate synthase]] | [[Category: Acetolactate synthase]] | ||
[[Category: Saccharomyces cerevisiae]] | [[Category: Saccharomyces cerevisiae]] | ||
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[[Category: thiamin diphosphate]] | [[Category: thiamin diphosphate]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:07:49 2008'' |
Revision as of 10:07, 20 March 2008
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| , resolution 2.6Å | |||||||
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| Ligands: | , , , and | ||||||
| Gene: | ILV2 (Saccharomyces cerevisiae) | ||||||
| Activity: | Acetolactate synthase, with EC number 2.2.1.6 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of the Catalytic Subunit of Yeast Acetohydroxyacid Synthase: A target for Herbicidal Inhibitors
Overview
Acetohydroxyacid synthase (AHAS; EC 4.1.3.18) catalyzes the first step in branched-chain amino acid biosynthesis. The enzyme requires thiamin diphosphate and FAD for activity, but the latter is unexpected, because the reaction involves no oxidation or reduction. Due to its presence in plants, AHAS is a target for sulfonylurea and imidazolinone herbicides. Here, the crystal structure to 2.6 A resolution of the catalytic subunit of yeast AHAS is reported. The active site is located at the dimer interface and is near the proposed herbicide-binding site. The conformation of FAD and its position in the active site are defined. The structure of AHAS provides a starting point for the rational design of new herbicides.
About this Structure
1JSC is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
Reference
Crystal structure of yeast acetohydroxyacid synthase: a target for herbicidal inhibitors., Pang SS, Duggleby RG, Guddat LW, J Mol Biol. 2002 Mar 22;317(2):249-62. PMID:11902841
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